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Literature summary extracted from
- Cellulases of Aspergillus aculeatus (1988), Methods Enzymol., 160, 274-299.
No PubMed abstract available
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 132000 | - |
beta-glucosidase 2, gel filtration | Aspergillus aculeatus |
| 3.2.1.21 | 133000 | - |
beta-glucosidase 1, gel filtration | Aspergillus aculeatus |
| 3.2.1.21 | 136000 | - |
beta-glucosidase 3, gel filtration | Aspergillus aculeatus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Aspergillus aculeatus | cellobiose | beta-D-glucose + D-glucose | - |
? |  |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Aspergillus aculeatus | low activity for beta-glucosidase 3 | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Aspergillus aculeatus NO. F-50 | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Aspergillus aculeatus NO. F-50 | low activity for beta-glucosidase 3 | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellohexaose + 5 H2O | Aspergillus aculeatus | low activity for beta-glucosidase 3 | 6 D-glucose | - |
? | |
| 3.2.1.21 | cellopentaose + 4 H2O | Aspergillus aculeatus | low activity for beta-glucosidase 3 | 5 D-glucose | - |
? | |
| 3.2.1.21 | cellotetraose + 3 H2O | Aspergillus aculeatus | low activity for beta-glucosidase 3 | 4 D-glucose | - |
? | |
| 3.2.1.21 | cellotriose + 2 H2O | Aspergillus aculeatus | low activity for beta-glucosidase 3 | 3 D-glucose | - |
? | |
| 3.2.1.21 | insoluble cellooligosaccharaide + H2O | Aspergillus aculeatus | beta-glucosidase 3 less active | beta-D-glucose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.4 | Aspergillus aculeatus | - |
NO. F-50 | - |
| 3.2.1.4 | Aspergillus aculeatus NO. F-50 | - |
NO. F-50 | - |
| 3.2.1.21 | Aspergillus aculeatus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | glycoprotein | - |
Aspergillus aculeatus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | - |
Aspergillus aculeatus |
| 3.2.1.21 | further purification of a cellulase preparation, chromatography steps, to homogeneity | Aspergillus aculeatus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | commercial preparation | - |
Aspergillus aculeatus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.4 | carboxymethylcellulose + H2O | - |
Aspergillus aculeatus | additional information | - |
? | |
| 3.2.1.4 | cellulose + H2O | acid-swollen | Aspergillus aculeatus | ? | - |
? | |
| 3.2.1.21 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Aspergillus aculeatus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | lower activity for beta-glucosidase 3 | Aspergillus aculeatus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Aspergillus aculeatus NO. F-50 | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | lower activity for beta-glucosidase 3 | Aspergillus aculeatus NO. F-50 | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus aculeatus | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus aculeatus NO. F-50 | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Aspergillus aculeatus | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity for beta-glucosidase 3 | Aspergillus aculeatus | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Aspergillus aculeatus NO. F-50 | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity for beta-glucosidase 3 | Aspergillus aculeatus NO. F-50 | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellohexaose + 5 H2O | low activity for beta-glucosidase 3 | Aspergillus aculeatus | 6 D-glucose | - |
? | |
| 3.2.1.21 | cellopentaose + 4 H2O | low activity for beta-glucosidase 3 | Aspergillus aculeatus | 5 D-glucose | - |
? | |
| 3.2.1.21 | cellotetraose + 3 H2O | low activity for beta-glucosidase 3 | Aspergillus aculeatus | 4 D-glucose | - |
? | |
| 3.2.1.21 | cellotriose + 2 H2O | low activity for beta-glucosidase 3 | Aspergillus aculeatus | 3 D-glucose | - |
? | |
| 3.2.1.21 | insoluble cellooligosaccharaide + H2O | beta-glucosidase 3 less active | Aspergillus aculeatus | beta-D-glucose + ? | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | low activity for beta-glucosidase 3 | Aspergillus aculeatus | beta-D-glucose + methanol | - |
? | |
| 3.2.1.21 | methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Aspergillus aculeatus | methylumbelliferone + beta-D-glucose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 55 | - |
beta-glucosidase 1 | Aspergillus aculeatus |
| 3.2.1.21 | 60 | - |
beta-glucosidase 2 | Aspergillus aculeatus |
| 3.2.1.21 | 65 | - |
beta-glucosidase 3 | Aspergillus aculeatus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
stable up to, beta-glucosidases 1 and 2 | Aspergillus aculeatus |
| 3.2.1.21 | 60 | - |
stable up to, beta-glucosidase 3 | Aspergillus aculeatus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 3 | - |
beta-glucosidase 3 | Aspergillus aculeatus |
| 3.2.1.21 | 4.5 | - |
beta-glucosidases 1 and 2 | Aspergillus aculeatus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 2.5 | 6 | stable within, beta-glucosidase 3 | Aspergillus aculeatus |
| 3.2.1.21 | 3 | 7 | stable within, beta-glucosidase 2 | Aspergillus aculeatus |
| 3.2.1.21 | 3.5 | 7 | stable within, beta-glucosidase 1 | Aspergillus aculeatus |
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