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Literature summary extracted from
- Purification of beta-D-glucosidase from Aspergillus niger (1988), Methods Enzymol., 160, 575-583.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.21 | stable to repeated freezing and thawing | Aspergillus niger |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.5 | - |
cellotriose | - |
Aspergillus niger |  |
| 3.2.1.21 | 0.51 | - |
cellotetraose | - |
Aspergillus niger | |
| 3.2.1.21 | 0.8 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Aspergillus niger | |
| 3.2.1.21 | 1.88 | - |
gentiobiose | - |
Aspergillus niger | |
| 3.2.1.21 | 1.89 | - |
cellobiose | - |
Aspergillus niger | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 118000 | - |
SDS-PAGE | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | (1-4)-beta-D-glucosyl-D-mannobiose + H2O | Aspergillus niger | - |
beta-D-glucose + D-mannobiose | - |
? | |
| 3.2.1.21 | (1-4)-beta-D-glucosyl-D-mannose + H2O | Aspergillus niger | - |
beta-D-glucose + D-mannose | - |
? | |
| 3.2.1.21 | 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Aspergillus niger | sophorose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 3(2)-beta-D-glucosyl-D-cellobiose + H2O | Aspergillus niger | - |
beta-D-glucose + D-cellobiose | - |
? | |
| 3.2.1.21 | 3-O-beta-cellobiosyl-D-glucose + H2O | Aspergillus niger | - |
beta-D-glucose + D-cellobiose | - |
? | |
| 3.2.1.21 | 3-O-beta-cellotriosyl-D-glucose + H2O | Aspergillus niger | - |
beta-D-glucose + D-cellotriose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Aspergillus niger | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Aspergillus niger | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellopentaose + 4 H2O | Aspergillus niger | - |
5 D-glucose | - |
? | |
| 3.2.1.21 | cellotetraose + 3 H2O | Aspergillus niger | - |
4 D-glucose | - |
? | |
| 3.2.1.21 | cellotriose + 2 H2O | Aspergillus niger | - |
3 D-glucose | - |
? | |
| 3.2.1.21 | gentiotriose + 2 H2O | Aspergillus niger | - |
3 D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Aspergillus niger | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | further purification of commercially available enzyme preparation, to homogeneity, chromatography steps | Aspergillus niger |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | commercial preparation | Novozym 188 | Aspergillus niger | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 52.8 | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | (1-4)-beta-D-glucosyl-D-mannobiose + H2O | - |
Aspergillus niger | beta-D-glucose + D-mannobiose | - |
? | |
| 3.2.1.21 | (1-4)-beta-D-glucosyl-D-mannose + H2O | - |
Aspergillus niger | beta-D-glucose + D-mannose | - |
? | |
| 3.2.1.21 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Aspergillus niger | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | (4-hydroxyphenyl)-beta-D-glucopyranoside + H2O | arbutin | Aspergillus niger | 4-hydroxyphenol + beta-D-glucopyranose | - |
? | |
| 3.2.1.21 | 2-nitrophenyl beta-D-galactopyranoside + H2O | very low activity | Aspergillus niger | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
| 3.2.1.21 | 2-nitrophenyl beta-D-xylopyranoside + H2O | very low activity | Aspergillus niger | 2-nitrophenol + beta-D-xylopyranose | - |
? | |
| 3.2.1.21 | 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | sophorose | Aspergillus niger | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 3(2)-beta-D-glucosyl-D-cellobiose + H2O | - |
Aspergillus niger | beta-D-glucose + D-cellobiose | - |
? | |
| 3.2.1.21 | 3-O-beta-cellobiosyl-D-glucose + H2O | - |
Aspergillus niger | beta-D-glucose + D-cellobiose | - |
? | |
| 3.2.1.21 | 3-O-beta-cellotriosyl-D-glucose + H2O | - |
Aspergillus niger | beta-D-glucose + D-cellotriose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus niger | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-mannopyranoside + H2O | very low activity | Aspergillus niger | 4-nitrophenol + beta-D-mannopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Aspergillus niger | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Aspergillus niger | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Aspergillus niger | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellopentaose + 4 H2O | - |
Aspergillus niger | 5 D-glucose | - |
? | |
| 3.2.1.21 | cellotetraose + 3 H2O | - |
Aspergillus niger | 4 D-glucose | - |
? | |
| 3.2.1.21 | cellotriose + 2 H2O | - |
Aspergillus niger | 3 D-glucose | - |
? | |
| 3.2.1.21 | esculin + H2O | - |
Aspergillus niger | beta-D-glucose + 6,7-dihydroxycoumarin | - |
? | |
| 3.2.1.21 | gentiobiose + H2O | - |
Aspergillus niger | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | gentiotriose + 2 H2O | - |
Aspergillus niger | 3 D-glucose | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | low activity | Aspergillus niger | beta-D-glucose + methanol | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 70 | 75 | - |
Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 65 | - |
stable up to | Aspergillus niger |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 2.5 | 9 | no loss of activity in 2 h at 4°C | Aspergillus niger |
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