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Literature summary extracted from
- Cellulase of Trichoderma koningii (1988), Methods Enzymol., 160, 221-233.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | D-glucono-1,5-lactone | beta-glucosidase 1, Ki: 1.8 mM, beta-glucosidase 2, Ki: 1.17 mM | Trichoderma koningii |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.37 | - |
2-Nitrophenyl-beta-D-glucopyranoside | beta-glucosidase 1 | Trichoderma koningii | |
| 3.2.1.21 | 0.85 | - |
2-Nitrophenyl-beta-D-glucopyranoside | beta-glucosidase 2 | Trichoderma koningii | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | extracellular | - |
Trichoderma koningii | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 13000 | - |
endoglucanase E1, gel filtration | Trichoderma koningii |
| 3.2.1.4 | 31000 | - |
endoglucanase E4, gel filtration | Trichoderma koningii |
| 3.2.1.4 | 48000 | - |
endoglucanase E3a and E3b, gel filtration | Trichoderma koningii |
| 3.2.1.21 | 39800 | - |
gel filtration | Trichoderma koningii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.4 | Trichoderma koningii | - |
- |
- |
| 3.2.1.21 | Trichoderma koningii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | - |
Trichoderma koningii |
| 3.2.1.21 | to homogeneity, chromatography steps, isoelectric focusing | Trichoderma koningii |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | cell culture | - |
Trichoderma koningii | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 47.8 | - |
beta-glucosidase 1 | Trichoderma koningii |
| 3.2.1.21 | 92.9 | - |
beta-glucosidase 2 | Trichoderma koningii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.4 | carboxymethylcellulose + H2O | - |
Trichoderma koningii | additional information | - |
? | |
| 3.2.1.4 | cellulose + H2O | acid-swollen | Trichoderma koningii | ? | - |
? | |
| 3.2.1.21 | 2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Trichoderma koningii | 2-nitrophenol + D-glucopyranose | - |
? | |
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Release 2023.1 (January 2023)
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