Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, H.K.; Pack, M.Y.
    Cloning and expression of Cellulomonas fimi beta-glucosidase genes in Escherichia coli (1989), Enzyme Microb. Technol., 11, 313-316.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Escherichia coli Cellulomonas fimi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Cellulomonas fimi cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Cellulomonas fimi only one of two cloned genes is active on cellobiose beta-D-glucose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Cellulomonas fimi
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 additional information chomosomal DNA, one gene coding for a cellobiose and aryl-glucoside hydrolysing enzyme, one gene coding for an only aryl-glucoside hydrolyzing enzyme Cellulomonas fimi
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 0.21
-
-
 Cellulomonas fimi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O one of two cloned genes are only active on this substrate Cellulomonas fimi 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Cellulomonas fimi beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O only one of two cloned genes is active on cellobiose Cellulomonas fimi beta-D-glucose + D-glucose
-
 ?