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Literature summary extracted from

  • Ong, E.; Gilkes, N.R.; Miller, R.C.; Warren, R.A.J.; Kilburn, D.G.
    Enzyme immobilization using a cellulose-binding domain: properties of a beta-glucosidase fusion protein (1991), Enzyme Microb. Technol., 13, 59-65.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Escherichia coli Agrobacterium sp.

General Stability

EC Number General Stability Organism
3.2.1.21 cellulose-binding domain-beta-glucosidase fusion protein: in solution stable for 24 h at 25°C, pH 7.0, if immobilized on cellulose: stable up to 7 weeks at 4°C or 37°C Agrobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Agrobacterium sp.
-
 fused enzyme to a cellulose-binding domain from Cex-exoglucanase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, as fusion protein by affinity chromatography Agrobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Agrobacterium sp. 4-nitrophenol + D-glucopyranose
-
 ?

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 5 9 rapid inactivation below and above for cellulose-immobilized Cex-cellulose-binding domain fused to beta-glucosidase Agrobacterium sp.