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Literature summary extracted from

  • Painbeni, E.; Valles, S.; Polaina, J.; Flors, A.
    Purification and characterization of a Bacillus polymyxa beta-glucosidase expressed in Escherichia coli (1992), J. Bacteriol., 174, 3087-3091.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Escherichia coli Paenibacillus polymyxa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 beta-D-glucose competitive inhibition, Ki: 19 mM Paenibacillus polymyxa
3.2.1.21 DTT 30% inhibition at 5 mM Paenibacillus polymyxa
3.2.1.21 Hg2+ strong inhibition at 1 mM Paenibacillus polymyxa
3.2.1.21 SDS 85% inhibition at 5 mM Paenibacillus polymyxa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.6
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Paenibacillus polymyxa
3.2.1.21 13
-
 cellobiose
-
 Paenibacillus polymyxa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 50000
-
 SDS-PAGE Paenibacillus polymyxa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Paenibacillus polymyxa cellobiose beta-D-glucose + D-glucose additional product: cellotriose by transglycosylation activity r

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Paenibacillus polymyxa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, recombinant enzyme Paenibacillus polymyxa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 additional information genomic library Paenibacillus polymyxa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 2417
-
-
 Paenibacillus polymyxa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Paenibacillus polymyxa 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Paenibacillus polymyxa beta-D-glucose + D-glucose additional product: cellotriose by transglycosylation activity r
3.2.1.21 cellobiose + H2O
-
 Paenibacillus polymyxa 2 beta-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 BglA
-
 Paenibacillus polymyxa