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Literature summary extracted from

  • Wright, R.M.; Yablonsky, M.D.; Shalita, Z.P.; Goyal, A.K.; Eveleigh, D.E.
    Cloning, characterization, and nucleotide sequence of a gene encoding Microbispora bispora BglB, a thermostable beta-glucosidase expressed in Escherichia coli (1992), Appl. Environ. Microbiol., 58, 3455-3465.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 beta-D-glucose cloned enzyme is activated 2-3fold at 0.1-0.3 M Thermobispora bispora

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Escherichia coli Thermobispora bispora

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 52280
-
 non-denaturating gradient PAGE Thermobispora bispora

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Thermobispora bispora cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Thermobispora bispora cloned enzyme show greater activity against cellobiose than against aryl-beta-D-glucosides beta-D-glucose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Thermobispora bispora
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, recombinant enzyme Thermobispora bispora

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 additional information genomic library Thermobispora bispora
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-methylumbelliferyl-beta-D-glucoside + H2O
-
 Thermobispora bispora 4-methylumbelliferone + beta-D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Thermobispora bispora beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cloned enzyme show greater activity against cellobiose than against aryl-beta-D-glucosides Thermobispora bispora beta-D-glucose + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 BglB
-
 Thermobispora bispora

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 60
-
-
 Thermobispora bispora

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 60
-
 retaining 70% of its activity after 48 h Thermobispora bispora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6.2
-
-
 Thermobispora bispora