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Literature summary extracted from

  • Drider, D.; Pommares, P.; Chemardin, P.; Arnaud, A.; Galzy, P.
    Purification and properties of the endocellular beta-glucosidase from Candida cacaoi Buckley and Van Uhden CBS 2020 (1993), J. Appl. Bacteriol., 74, 473-479.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Ag2+ strong inhibition at 10 mM Millerozyma farinosa
3.2.1.21 beta-D-glucose competitive inhibition, Ki: 8 mM Millerozyma farinosa
3.2.1.21 Cu2+ moderate inhibition at 10 mM Millerozyma farinosa
3.2.1.21 Fe3+ moderate inhibition at 10 mM Millerozyma farinosa
3.2.1.21 Hg2+ strong inhibition at 10 mM Millerozyma farinosa
3.2.1.21 Mg2+ moderate inhibition at 10 mM Millerozyma farinosa
3.2.1.21 Mn2+ moderate inhibition at 10 mM Millerozyma farinosa
3.2.1.21 N-bromosuccinimide inhibition at 10 mM Millerozyma farinosa
3.2.1.21 p-chloromercuribenzoic acid inhibition at 1 mM Millerozyma farinosa
3.2.1.21 Ru2+ moderate inhibition at 10 mM Millerozyma farinosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.44
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Millerozyma farinosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 intracellular stimulation of enzyme synthesis by cellobiose as carbohydrate source Millerozyma farinosa 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 Fe2+ activation at 10 mM Millerozyma farinosa
3.2.1.21 Na+ activation at 10 mM Millerozyma farinosa
3.2.1.21 Ni2+ activation at 10 mM Millerozyma farinosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 110000
-
 2 * 110000, SDS-PAGE Millerozyma farinosa
3.2.1.21 220000
-
 gel filtration Millerozyma farinosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Millerozyma farinosa low activity beta-D-glucose + D-glucose
-
 ?
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Millerozyma farinosa sophorose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Millerozyma farinosa laminaribiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Millerozyma farinosa cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Millerozyma farinosa low activity beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O Millerozyma farinosa low activity beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O Millerozyma farinosa gentiobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 alpha-L-arabinofuranose + H2O Millerozyma farinosa low activity alpha-L-arabinofuranose + ?
-
 ?
3.2.1.21 alpha-L-rhamnopyranoside + H2O Millerozyma farinosa
-
 alpha-L-rhamnopyranose + ?
-
 ?
3.2.1.21 trehalose + H2O Millerozyma farinosa
-
 beta-D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Millerozyma farinosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, 2step chromatography Millerozyma farinosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture
-
 Millerozyma farinosa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 38.63
-
-
 Millerozyma farinosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Millerozyma farinosa 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.21 (4-hydroxyphenyl)-beta-D-glucopyranoside + H2O arbutin Millerozyma farinosa 4-hydroxyphenol + beta-D-glucopyranose
-
 ?
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Millerozyma farinosa 2-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O low activity Millerozyma farinosa beta-D-glucose + D-glucose
-
 ?
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Millerozyma farinosa beta-D-glucose + D-glucose
-
 ?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Millerozyma farinosa beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-fucoside + H2O
-
 Millerozyma farinosa 4-nitrophenol + beta-D-fucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactoside + H2O
-
 Millerozyma farinosa 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Millerozyma farinosa 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl-alpha-D-glucoside
-
 Millerozyma farinosa 4-nitrophenol + alpha-D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Millerozyma farinosa beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Millerozyma farinosa beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Millerozyma farinosa beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Millerozyma farinosa beta-D-glucose + D-glucose
-
 ?
3.2.1.21 alpha-L-arabinofuranose + H2O low activity Millerozyma farinosa alpha-L-arabinofuranose + ?
-
 ?
3.2.1.21 alpha-L-rhamnopyranoside + H2O
-
 Millerozyma farinosa alpha-L-rhamnopyranose + ?
-
 ?
3.2.1.21 methyl-beta-D-glucoside + H2O low activity Millerozyma farinosa beta-D-glucose + methanol
-
 ?
3.2.1.21 trehalose + H2O
-
 Millerozyma farinosa beta-D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 dimer 2 * 110000, SDS-PAGE Millerozyma farinosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 60
-
-
 Millerozyma farinosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5.5
-
-
 Millerozyma farinosa