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Literature summary extracted from

  • Leah, R.; Kigel, J.; Svendson, I.; Mundy, J.
    Biochemical and molecular characterization of a barley seed beta-glucosidase (1995), J. Biol. Chem., 270, 15789-15797.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 3
-
 laminaribiose
-
 Hordeum vulgare
3.2.1.21 4.5
-
 cellobiose
-
 Hordeum vulgare
3.2.1.21 6.3
-
 sophorose
-
 Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 60000
-
 SDS-PAGE Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Hordeum vulgare sophorose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Hordeum vulgare specific for beta-1,2-, -1,3- or -1,4-hydrolysis beta-D-glucose + D-glucose
-
 ?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Hordeum vulgare laminaribiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Hordeum vulgare cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Hordeum vulgare complete hydrolsis of barley endosperm cell wall through synergistic action of endo(1-3,1-4)-beta-glucanase and beta-glucosidase beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellopentaose + 4 H2O Hordeum vulgare
-
 5 D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O Hordeum vulgare
-
 4 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O Hordeum vulgare
-
 3 D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, 2step chromatography Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 seed 3 isoforms Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O low activity Hordeum vulgare 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Hordeum vulgare 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Hordeum vulgare beta-D-glucose + D-glucose
-
 ?
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O specific for beta-1,2-, -1,3- or -1,4-hydrolysis Hordeum vulgare beta-D-glucose + D-glucose
-
 ?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Hordeum vulgare beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Hordeum vulgare 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-mannopyranoside + H2O
-
 Hordeum vulgare 4-nitrophenol + beta-D-mannopyranose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Hordeum vulgare beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O complete hydrolsis of barley endosperm cell wall through synergistic action of endo(1-3,1-4)-beta-glucanase and beta-glucosidase Hordeum vulgare beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Hordeum vulgare 2 beta-D-glucose
-
 ?
3.2.1.21 cellopentaose + 4 H2O
-
 Hordeum vulgare 5 D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O
-
 Hordeum vulgare 4 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O
-
 Hordeum vulgare 3 D-glucose
-
 ?
3.2.1.21 laminaribiose + H2O
-
 Hordeum vulgare 2 beta-D-glucose
-
 ?
3.2.1.21 salicylic acid-beta-D-glucoside + H2O low activity Hordeum vulgare beta-D-glucose + salicylate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 60
-
-
 Hordeum vulgare

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 50
-
 half life: 6 min Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5
-
-
 Hordeum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 4.3 6.5 more than 80% of activity within Hordeum vulgare