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Literature summary extracted from

  • Yang, L.; Akao, T.; Kobashi, K.
    Purification and characterization of a geniposide-hydrolyzing beta-glucosidase from Eubacterium sp. A-44, a strict anaerobe from human feces (1995), Biol. Pharm. Bull., 18, 1175-1178.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 5,5'-dithiobis(2-nitrobenzoic acid) complete inhibition at 0.2 mM Eubacterium sp.
3.2.1.21 Cu2+ strong inhibition at 0.1 mM Eubacterium sp.
3.2.1.21 nojirimycin bisulfite strong inhibition at 0.012 mM Eubacterium sp.
3.2.1.21 p-chloromercuribenzene sulfonic acid complete inhibition at 0.02 mM Eubacterium sp.
3.2.1.21 p-chloromercuribenzoic acid strong inhibition at 0.1 mM Eubacterium sp.
3.2.1.21 Zn2+ slight inhibition at 1 mM Eubacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.068
-
 geniposide
-
 Eubacterium sp.
3.2.1.21 0.1
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Eubacterium sp.
3.2.1.21 0.21
-
 esculin
-
 Eubacterium sp.
3.2.1.21 0.22
-
 Salicin
-
 Eubacterium sp.
3.2.1.21 0.91
-
 cellobiose
-
 Eubacterium sp.
3.2.1.21 2.9
-
 amygdalin
-
 Eubacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 intracellular
-
 Eubacterium sp. 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 90000
-
 gel filtration Eubacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Eubacterium sp. cellobiose beta-D-glucose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Eubacterium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, chromatography steps Eubacterium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture
-
 Eubacterium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 24.7
-
 for 4-nitrophenyl-beta-D-glucopyranoside Eubacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Eubacterium sp. 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.21 (R)-alpha-[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]benzeneacetonitrile + H2O amygdalin Eubacterium sp. HCN + ?
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Eubacterium sp. 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Eubacterium sp. beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Eubacterium sp. 2 beta-D-glucose
-
 ?
3.2.1.21 esculin + H2O
-
 Eubacterium sp. beta-D-glucose + 6,7-dihydroxycoumarin
-
 ?
3.2.1.21 geniposide + H2O
-
 Eubacterium sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 90000, SDS-PAGE Eubacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6
-
-
 Eubacterium sp.