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Literature summary extracted from

  • Skory, C.D.; Freer, S.N.; Bothast, R.J.
    Properties of an intracellular beta-glucosidase purified from the cellobiose-fermenting yeast Candida wickerhamii (1996), Appl. Microbiol. Biotechnol., 546, 353-359.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 1-butanol 1.6fold increase of activity Nakazawaea wickerhamii
3.2.1.21 1-Pentanol 1.6fold increase of activity Nakazawaea wickerhamii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 2-butanol highest decrease of activity in comparison to ethanol and 2-propanol Nakazawaea wickerhamii
3.2.1.21 2-propanol higher decrease of activity than for ethanol Nakazawaea wickerhamii
3.2.1.21 beta-D-glucose end-product inhibition, 42% inhibition at 100 mM Nakazawaea wickerhamii
3.2.1.21 ethanol decrease of activity Nakazawaea wickerhamii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.28
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Nakazawaea wickerhamii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 intracellular stimulation of enzyme synthesis by cellobiose as carbohydrate source Nakazawaea wickerhamii 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 94000
-
 2 * 94000, SDS-PAGE Nakazawaea wickerhamii
3.2.1.21 180000
-
 gel filtration Nakazawaea wickerhamii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O Nakazawaea wickerhamii lactose beta-D-galactose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Nakazawaea wickerhamii cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 trehalose + H2O Nakazawaea wickerhamii
-
 beta-D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Nakazawaea wickerhamii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, chromatography steps Nakazawaea wickerhamii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture
-
 Nakazawaea wickerhamii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 10.1
-
-
 Nakazawaea wickerhamii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O highest activity against aryl-beta-1,4-glucosides Nakazawaea wickerhamii 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Nakazawaea wickerhamii 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O lactose Nakazawaea wickerhamii beta-D-galactose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Nakazawaea wickerhamii beta-D-glucose + D-glucose
-
 ?
3.2.1.21 trehalose + H2O
-
 Nakazawaea wickerhamii beta-D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 dimer 2 * 94000, SDS-PAGE Nakazawaea wickerhamii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 35
-
-
 Nakazawaea wickerhamii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 28
-
 labile above Nakazawaea wickerhamii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6 6.5
-
 Nakazawaea wickerhamii