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Literature summary extracted from

  • Pitson, S.M.; Seviour, R.J.; McDougall, B.M.
    Purification and characterization of an extracellular beta-glucosidase from the filamentous fungus Acremonium persicinum and its probable role in beta-glucan degradation (1997), Enzyme Microb. Technol., 21, 182-190.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 2-Hydroxy-5-nitrobenzyl bromide strong inhibition at 10 mM Acremonium persicinum
3.2.1.21 beta-D-glucose competitive inhibition, Ki: 0.35 mM Acremonium persicinum
3.2.1.21 Cu2+ slight inhibition at 1 mM Acremonium persicinum
3.2.1.21 D-glucono-1,5-lactone competitive inhibition, Ki: 0.005 mM Acremonium persicinum
3.2.1.21 dicyclohexyl carbodiimide nearly complete inhibition at 10 mM Acremonium persicinum
3.2.1.21 Hg2+ slight inhibition at 1 mM Acremonium persicinum
3.2.1.21 KMnO4 92.7% inhibition at 1 mM Acremonium persicinum
3.2.1.21 N-Acetylimidazole strong inhibition at 10 mM Acremonium persicinum
3.2.1.21 N-bromosuccinimide complete inhibition at 1 mM Acremonium persicinum
3.2.1.21 N-ethyl-5-phenylisoxazolium-3'-sulfonate nearly complete inhibition at 2 mM Acremonium persicinum
3.2.1.21 Zn2+ 56% inhibition at 1 mM Acremonium persicinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.3
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Acremonium persicinum
3.2.1.21 0.63
-
 laminaribiose
-
 Acremonium persicinum
3.2.1.21 0.7
-
 gentiobiose
-
 Acremonium persicinum
3.2.1.21 0.91
-
 cellobiose
-
 Acremonium persicinum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular
-
 Acremonium persicinum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 Mg2+ slight activation at 1 mM Acremonium persicinum
3.2.1.21 Sn2+ slight activation at 1 mM Acremonium persicinum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 128000
-
 SDS-PAGE, gel filtration Acremonium persicinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Acremonium persicinum sophorose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Acremonium persicinum laminaribiose beta-D-glucose + D-glucose
-
 r
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Acremonium persicinum high activity on short oligosaccharides, decreasing activity for higher oligosaccharides, transglycosylation reaction beta-D-glucose + D-glucose
-
 r
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Acremonium persicinum cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Acremonium persicinum high activity on short oligosaccharides, decreasing activity for higher oligosaccharides beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O Acremonium persicinum gentiobiose beta-D-glucose + D-glucose
-
 r
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O Acremonium persicinum transglycosylation reaction beta-D-glucose + D-glucose
-
 r
3.2.1.21 celloheptaose + 6 H2O Acremonium persicinum low activity 7 D-glucopyranose
-
 ?
3.2.1.21 cellohexaose + 5 H2O Acremonium persicinum low activity 6 D-glucose
-
 ?
3.2.1.21 cellopentaose + 4 H2O Acremonium persicinum
-
 5 D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O Acremonium persicinum
-
 4 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O Acremonium persicinum
-
 3 D-glucose
-
 ?
3.2.1.21 laminariheptaose + 6 H2O Acremonium persicinum
-
 7 beta-D-glucose
-
 ?
3.2.1.21 laminarihexaose + 5 H2O Acremonium persicinum
-
 6 D-glucose
-
 ?
3.2.1.21 laminaripentaose + 4 H2O Acremonium persicinum
-
 5 D-glucose
-
 ?
3.2.1.21 laminaritetraose + 3 H2O Acremonium persicinum
-
 4 D-glucose
-
 ?
3.2.1.21 laminaritriose + 2 H2O Acremonium persicinum
-
 3 D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Acremonium persicinum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, chromatography steps Acremonium persicinum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture
-
 Acremonium persicinum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 255
-
-
 Acremonium persicinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Acremonium persicinum 2-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Acremonium persicinum beta-D-glucose + D-glucose
-
 ?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Acremonium persicinum beta-D-glucose + D-glucose
-
 r
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O high activity on short oligosaccharides, decreasing activity for higher oligosaccharides, transglycosylation reaction Acremonium persicinum beta-D-glucose + D-glucose
-
 r
3.2.1.21 4-methylumbelliferyl-beta-D-glucoside + H2O
-
 Acremonium persicinum 4-methylumbelliferone + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-cellobioside + 2 H2O very low activity Acremonium persicinum 4-nitrophenol + 2 beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O implicated in beta-glucan degradation through synergistic action with beta-glucanases Acremonium persicinum 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O very low activity Acremonium persicinum 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Acremonium persicinum beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O high activity on short oligosaccharides, decreasing activity for higher oligosaccharides Acremonium persicinum beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Acremonium persicinum beta-D-glucose + D-glucose
-
 r
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O transglycosylation reaction Acremonium persicinum beta-D-glucose + D-glucose
-
 r
3.2.1.21 cellobiose + H2O
-
 Acremonium persicinum 2 beta-D-glucose
-
 ?
3.2.1.21 celloheptaose + 6 H2O low activity Acremonium persicinum 7 D-glucopyranose
-
 ?
3.2.1.21 cellohexaose + 5 H2O low activity Acremonium persicinum 6 D-glucose
-
 ?
3.2.1.21 cellopentaose + 4 H2O
-
 Acremonium persicinum 5 D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O
-
 Acremonium persicinum 4 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O
-
 Acremonium persicinum 3 D-glucose
-
 ?
3.2.1.21 gentiobiose + H2O
-
 Acremonium persicinum 2 beta-D-glucose
-
 ?
3.2.1.21 laminaribiose + H2O
-
 Acremonium persicinum 2 beta-D-glucose
-
 ?
3.2.1.21 laminariheptaose + 6 H2O
-
 Acremonium persicinum 7 beta-D-glucose
-
 ?
3.2.1.21 laminarihexaose + 5 H2O
-
 Acremonium persicinum 6 D-glucose
-
 ?
3.2.1.21 laminaripentaose + 4 H2O
-
 Acremonium persicinum 5 D-glucose
-
 ?
3.2.1.21 laminaritetraose + 3 H2O
-
 Acremonium persicinum 4 D-glucose
-
 ?
3.2.1.21 laminaritriose + 2 H2O
-
 Acremonium persicinum 3 D-glucose
-
 ?
3.2.1.21 phenyl-beta-D-glucoside + H2O low activity Acremonium persicinum phenol + beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 128000, SDS-PAGE Acremonium persicinum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 640
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Acremonium persicinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5.5
-
-
 Acremonium persicinum