EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.B26 | analysis | reporter genes in molecular biology | Saccharolobus solfataricus |
3.2.1.B26 | nutrition | catalyst of lactose hydrolysis in dairy products in the food industry | Saccharolobus solfataricus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.B26 | expressed in Saccharomyces cerevisiae | Saccharolobus solfataricus |
3.2.1.B26 | expression in Saccharomyces cerevisiae | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B26 | 0.3 | - |
4-nitrophenyl-cellobioside | - |
Saccharolobus solfataricus | |
3.2.1.B26 | 0.3 | - |
4-nitrophenyl cellobioside | pH 6.5, 75°C, recombinant enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 0.3 | - |
4-nitrophenyl cellobioside | pH 6.5, 75°C, wild-type enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 0.5 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Saccharolobus solfataricus | |
3.2.1.B26 | 0.5 | - |
4-nitrophenyl beta-D-glucoside | pH 6.5, 75°C, recombinant enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 0.5 | - |
4-nitrophenyl beta-D-glucoside | pH 6.5, 75°C, wild-type enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 0.7 | - |
4-nitrophenyl-beta-D-fucoside | - |
Saccharolobus solfataricus | |
3.2.1.B26 | 0.7 | - |
4-nitrophenyl beta-D-fucoside | pH 6.5, 75°C, recombinant enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 1 | - |
4-nitrophenyl beta-D-fucoside | pH 6.5, 75°C, wild-type enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 1.4 | - |
laminaribiose | - |
Saccharolobus solfataricus | |
3.2.1.B26 | 3.9 | - |
2-nitrophenyl-beta-D-galactopyranoside | - |
Saccharolobus solfataricus | |
3.2.1.B26 | 3.9 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 6.5, 75°C, recombinant enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 4 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 6.5, 75°C, wild-type enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 4.3 | - |
4-nitrophenyl-beta-D-galactopyranoside | - |
Saccharolobus solfataricus | |
3.2.1.B26 | 4.3 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.5, 75°C, recombinant enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 4.7 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.5, 75°C, wild-type enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 31.5 | - |
cellobiose | - |
Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.B26 | 60000 | - |
4 * 60000, SDS-PAGE | Saccharolobus solfataricus |
3.2.1.B26 | 240000 | - |
gel filtration | Saccharolobus solfataricus |
3.2.1.B26 | 240000 | - |
gel filtration, glycerol gradient centrifugation | Saccharolobus solfataricus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.B26 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Saccharolobus solfataricus | laminaribiose | beta-D-glucose + D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.B26 | Saccharolobus solfataricus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.B26 | - |
Saccharolobus solfataricus |
3.2.1.B26 | to homogeneity, recombinant enzyme | Saccharolobus solfataricus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.B26 | 166 | - |
- |
Saccharolobus solfataricus |
3.2.1.B26 | 166 | - |
pH 6.5, 75°C | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.B26 | 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
3.2.1.B26 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Saccharolobus solfataricus | beta-D-glucose + D-glucose | - |
? | |
3.2.1.B26 | 4-nitrophenyl beta-cellobioside + 2 H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + 2 beta-D-glucose | - |
? | |
3.2.1.B26 | 4-nitrophenyl beta-D-fucoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-fucose | - |
? | |
3.2.1.B26 | 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
3.2.1.B26 | 4-nitrophenyl beta-D-galactoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.B26 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + D-glucopyranose | - |
? | |
3.2.1.B26 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.B26 | 4-nitrophenyl cellobioside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + cellobiose | - |
? | |
3.2.1.B26 | additional information | hydrolase with broad specificity and noticeable glucan 1 -> 4-beta-D-glucosidase activity | Saccharolobus solfataricus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.B26 | tetramer | 4 * 60000, SDS-PAGE | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.B26 | Sbetagly | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.B26 | 80 | - |
steady increase in activity up to 80°C | Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.B26 | 75 | - |
5 h, 17% loss of activity | Saccharolobus solfataricus |
3.2.1.B26 | 85 | - |
83% residual activity after 5 h | Saccharolobus solfataricus |
3.2.1.B26 | 85 | - |
5 h, 43% loss of activity | Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B26 | 448 | - |
4-nitrophenyl cellobioside | pH 6.5, 75°C, recombinant enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 503 | - |
4-nitrophenyl beta-D-fucoside | pH 6.5, 75°C, recombinant enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 503 | - |
4-nitrophenyl cellobioside | pH 6.5, 75°C, wild-type enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 542 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Saccharolobus solfataricus | |
3.2.1.B26 | 542 | - |
4-nitrophenyl beta-D-glucoside | pH 6.5, 75°C, recombinant enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 542 | - |
4-nitrophenyl beta-D-glucoside | pH 6.5, 75°C, wild-type enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 772 | - |
4-nitrophenyl beta-D-fucoside | pH 6.5, 75°C, wild-type enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 1020 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.5, 75°C, wild-type enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 1239 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 6.5, 75°C, wild-type enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 1285 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.5, 75°C, recombinant enzyme | Saccharolobus solfataricus | |
3.2.1.B26 | 1713 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 6.5, 75°C, recombinant enzyme | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.B26 | 7 | - |
- |
Saccharolobus solfataricus |
3.2.1.B26 | 7 | - |
in 50 mM sodium phosphate buffer, substrate: 2-nitrophenyl beta-D-galactopyranoside | Saccharolobus solfataricus |
3.2.1.B26 | 7 | - |
in 50 mM sodium phosphate buffer, substrate: 2-nitrophenyl-beta-D-galactopyranoside | Saccharolobus solfataricus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.B26 | 5.5 | 8.5 | pH 5.5: about 50% of maximal activity, pH 8.5: about 50% of maximal activity | Saccharolobus solfataricus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.B26 | Saccharolobus solfataricus | isoelectric focusing | - |
4 |