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Literature summary extracted from

  • Abdel-Naby, M.A.; Osman, M.Y.; Abdel-Fattah, A.F.
    Purification and properties of three cellobiases from Aspergillus niger A20 (1999), Appl. Biochem. Biotechnol., 76, 33-44.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Ba2+ slight inhibition at 5-10 mM Aspergillus niger
3.2.1.21 Cd2+ slight inhibition at 5-10 mM Aspergillus niger
3.2.1.21 Cu2+ slight inhibition at 5-10 mM Aspergillus niger
3.2.1.21 Hg2+ complete inhibition at 5 mM Aspergillus niger
3.2.1.21 Iodine complete inactivation at 5 mM Aspergillus niger
3.2.1.21 Zn2+ slight inhibition at 5-10 mM Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.9
-
 cellobiose for cellobiase A Aspergillus niger
3.2.1.21 1
-
 cellobiose for cellobiase C Aspergillus niger
3.2.1.21 1.36
-
 cellobiose cellobiase B Aspergillus niger
3.2.1.21 1.4
-
 2-Nitrophenyl-beta-D-glucopyranoside
-
 Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular
-
 Aspergillus niger
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 Ca2+ stimulation of cellobiases B and C at 5-10 mM Aspergillus niger
3.2.1.21 Co2+ stimulation for cellobiase A at 5-10 mM Aspergillus niger
3.2.1.21 Mg2+ stimulation of cellobiase A at 5-10 mM Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 71000
-
 cellobiase C, SDS-PAGE Aspergillus niger
3.2.1.21 80000
-
 cellobiase B, SDS-PAGE Aspergillus niger
3.2.1.21 88000
-
 cellobiase A, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O Aspergillus niger low activity beta-D-galactose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O Aspergillus niger lactose beta-D-galactose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus niger cellobiose beta-D-glucose + D-glucose additional product: cellotetraose as the product of transglycosylation r
3.2.1.21 dextran + H2O Aspergillus niger very low activity beta-D-glucose + ?
-
 ?
3.2.1.21 starch + H2O Aspergillus niger low activity beta-D-glucose + ?
-
 ?
3.2.1.21 xylan + H2O Aspergillus niger very low activity beta-D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.21 glycoprotein carbohydrate content, w/w: 8.8 cellobiase A, 9.4 cellobiase B, 7.2 cellobiase C Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, chromatography steps Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture 3 isoenzmes, A, B and C Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 119.3
-
 cellobiase B Aspergillus niger
3.2.1.21 140.4
-
 cellobiase C Aspergillus niger
3.2.1.21 184.1
-
 cellobiase A Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-galactoside + H2O low activity Aspergillus niger 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O best substrate Aspergillus niger 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O low activity Aspergillus niger 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O low activity Aspergillus niger beta-D-galactose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O lactose Aspergillus niger beta-D-galactose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Aspergillus niger beta-D-glucose + D-glucose additional product: cellotetraose as the product of transglycosylation r
3.2.1.21 carboxymethyl-cellulose + H2O low activity Aspergillus niger beta-D-glucose + ?
-
 ?
3.2.1.21 dextran + H2O very low activity Aspergillus niger beta-D-glucose + ?
-
 ?
3.2.1.21 starch + H2O low activity Aspergillus niger beta-D-glucose + ?
-
 ?
3.2.1.21 xylan + H2O very low activity Aspergillus niger beta-D-glucose + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 55
-
 cellobiases A, B Aspergillus niger
3.2.1.21 60
-
 cellobiase C Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 55
-
 half-life of cellobiase A: 27 min, cellobiase B: 32.3 min Aspergillus niger
3.2.1.21 60
-
 half-life for cellobiase C: 45 min Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 4
-
-
 Aspergillus niger
3.2.1.21 4.5
-
 cellobiases A, B, C Aspergillus niger