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Literature summary extracted from

  • Dion, M.; Fourage, L.; Hallet, J.N.; Colas, B.
    Cloning and expression of a beta-glycosidase gene from Thermus thermophilus. Sequence and biochemical characterization of the encoded enzyme (1999), Glycoconj. J., 16, 27-37.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Escherichia coli Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Tris inhibitory effect Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.09
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Thermus thermophilus
3.2.1.21 0.16
-
 4-nitrophenyl-beta-D-fucoside
-
 Thermus thermophilus
3.2.1.21 4
-
 cellobiose
-
 Thermus thermophilus
3.2.1.21 6.6
-
 4-nitrophenyl-beta-D-galactoside
-
 Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 48600
-
 gel filtration Thermus thermophilus
3.2.1.21 48642
-
 1 * 48642, deduced from amino acid composition, SDS-PAGE Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Thermus thermophilus sophorose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Thermus thermophilus laminaribiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Thermus thermophilus preference for beta-1,3-bonds beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O Thermus thermophilus lactose beta-D-galactose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Thermus thermophilus cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O Thermus thermophilus low activity beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O Thermus thermophilus gentiobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellopentaose + 4 H2O Thermus thermophilus
-
 5 D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O Thermus thermophilus
-
 4 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O Thermus thermophilus
-
 3 D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, recombinant enzyme Thermus thermophilus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 additional information genomic library Thermus thermophilus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermus thermophilus 2-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.21 2-nitrophenyl beta-D-galactopyranoside 6-phosphate + H2O
-
 Thermus thermophilus 2-nitrophenol + beta-D-galactopyranose 6-phosphate
-
 ?
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermus thermophilus 2-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 2-nitrophenyl-beta-D-fucoside + H2O
-
 Thermus thermophilus 2-nitrophenol + beta-D-fucose
-
 ?
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Thermus thermophilus beta-D-glucose + D-glucose
-
 ?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Thermus thermophilus beta-D-glucose + D-glucose
-
 ?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O preference for beta-1,3-bonds Thermus thermophilus beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-fucoside + 2-phenylethanol best substrate for transglycolsylation reaction Thermus thermophilus 2-phenylethyl-beta-D-fucoside + 4-nitrophenol
-
 r
3.2.1.21 4-nitrophenyl beta-D-fucoside + H2O
-
 Thermus thermophilus 4-nitrophenol + beta-D-fucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactoside + 2-phenylethanol transglycosylation reaction Thermus thermophilus 2-phenylethyl beta-D-galactoside + 4-nitrophenol
-
 r
3.2.1.21 4-nitrophenyl beta-D-galactoside + H2O
-
 Thermus thermophilus 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermus thermophilus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucoside + 2-phenylethanol transglycosylation reaction Thermus thermophilus 2-phenylethyl-beta-D-glucoside + 4-nitrophenol
-
 r
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O low activity Thermus thermophilus 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.21 4-O-beta-D-galactopyranosyl-D-glucose + H2O lactose Thermus thermophilus beta-D-galactose + D-glucose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Thermus thermophilus beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Thermus thermophilus beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Thermus thermophilus beta-D-glucose + D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Thermus thermophilus 2 beta-D-glucose
-
 ?
3.2.1.21 cellopentaose + 4 H2O
-
 Thermus thermophilus 5 D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O
-
 Thermus thermophilus 4 D-glucose
-
 ?
3.2.1.21 cellotriose + 2 H2O
-
 Thermus thermophilus 3 D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 48642, deduced from amino acid composition, SDS-PAGE Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 88
-
 for 4-nitrophenyl-beta-D-glucoside Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 80
-
 stable up to Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 23
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6.5 7
-
 Thermus thermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 6 7.5 retaining more than 80% of the activity Thermus thermophilus