EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.21 | synthesis | synthesis of glycoconjugates and oligosaccharides | Pyrococcus furiosus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.21 | expression in Escherichia coli | Pyrococcus furiosus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.21 | 3.3 A resolution | Pyrococcus furiosus |
3.2.1.B28 | 3.3 A resolution structural model. CelB shows a homo-tetramer configuration, with subunits having a typical (betaalpha)8-barrel fold | Pyrococcus furiosus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.21 | E417S | mutant with reduced pH optimum, increased Km and turnover number for 2-nitrophenyl-beta-1,4-D-glucopyranoside | Pyrococcus furiosus |
3.2.1.21 | F426Y | mutant with unchanged pH optimum, slight increased Km and slight reduced turnover number for 2-nitrophenyl-beta-1,4-D-glucopyranoside | Pyrococcus furiosus |
3.2.1.21 | M424K | mutant with increased pH optimum, slight increased Km and reduced turnover number for 2-nitrophenyl-beta-1,4-D-galactopyranoside | Pyrococcus furiosus |
3.2.1.B28 | E417S | mutation in phosphate binding site, 5fold increase of the efficiency of hydrolyzing o-nitrophenol-beta-D-galactopyranoside-6-phosphate. Activity on nonphosphorylated sugars is largely reduced | Pyrococcus furiosus |
3.2.1.B28 | E417S/M424K/F426Y | mutations in phosphate binding site, 3fold increase of the efficiency of hydrolyzing 2-nitrophenyl beta-D-galactopyranoside-6-phosphate. Activity on nonphosphorylated sugars is largely reduced | Pyrococcus furiosus |
3.2.1.B28 | F426Y | mutation in phosphate binding site, results in an increased affinity for galactosides. Activity on nonphosphorylated sugars is largely reduced | Pyrococcus furiosus |
3.2.1.B28 | M424K | mutation in phosphate binding site, shifting pH optimum from 5.0 to 6.0. Activity on nonphosphorylated sugars is largely reduced | Pyrococcus furiosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 0.28 | - |
2-Nitrophenyl-beta-D-glucopyranoside | - |
Pyrococcus furiosus | |
3.2.1.21 | 2.3 | - |
2-nitrophenyl-beta-D-galactopyranoside | - |
Pyrococcus furiosus | |
3.2.1.21 | 31 | - |
2-nitrophenyl-beta-D-galactopyranoside-6-phosphate | - |
Pyrococcus furiosus | |
3.2.1.B28 | 0.28 | - |
2-nitrophenyl beta-D-glucopyranoside | wild-type, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 0.51 | - |
2-nitrophenyl beta-D-glucopyranoside | mutant M424K, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 0.57 | - |
2-nitrophenyl beta-D-glucopyranoside | mutant F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 0.89 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 2.3 | - |
2-nitrophenyl beta-D-galactopyranoside | wild-type, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 4.8 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant M424K, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 16 | - |
2-nitrophenyl beta-D-galactopyranoside-6-phosphate | mutant E417S/M424K/F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 22 | - |
2-nitrophenyl beta-D-galactopyranoside-6-phosphate | mutant E417S, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 29 | - |
2-nitrophenyl beta-D-glucopyranoside | mutant E417S, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 31 | - |
2-nitrophenyl beta-D-galactopyranoside-6-phosphate | wild-type, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 38 | - |
2-nitrophenyl beta-D-galactopyranoside-6-phosphate | mutant F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 40 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant E417S/M424K/F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 42 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant E417S, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 59 | - |
2-nitrophenyl beta-D-glucopyranoside | mutant E417S/M424K/F426Y, pH 5.0, 90°C | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Pyrococcus furiosus | Q51723 | - |
- |
3.2.1.B28 | Pyrococcus furiosus | Q51723 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.21 | to homogeneity, recombinant enzyme | Pyrococcus furiosus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.21 | additional information | celB gene | Pyrococcus furiosus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 2-nitrophenyl beta-1,4-D-galactopyranoside 6-phosphate + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
3.2.1.21 | 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
3.2.1.21 | 2-nitrophenyl beta-D-galactopyranoside 6-phosphate + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + beta-D-galactopyranose 6-phosphate | - |
? | |
3.2.1.21 | 2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + D-glucopyranose | - |
? | |
3.2.1.B28 | 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + D-galactopyranoside | - |
? | |
3.2.1.B28 | 2-nitrophenyl beta-D-galactopyranoside-6-phosphate + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + D-galactopyranoside-6-phosphate | - |
? | |
3.2.1.B28 | 2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + D-glucopyranoside | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.21 | tetramer | composed of 4 identical subunits, X-ray diffraction study | Pyrococcus furiosus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.21 | CelB | - |
Pyrococcus furiosus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 49 | - |
2-nitrophenyl beta-1,4-D-galactopyranoside 6-phosphate | - |
Pyrococcus furiosus | |
3.2.1.21 | 860 | - |
2-Nitrophenyl-beta-D-glucopyranoside | - |
Pyrococcus furiosus | |
3.2.1.21 | 2050 | - |
2-nitrophenyl-beta-D-galactopyranoside | - |
Pyrococcus furiosus | |
3.2.1.B28 | 20 | 50 | 2-nitrophenyl beta-D-galactopyranoside | wild-type, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 26 | - |
2-nitrophenyl beta-D-galactopyranoside-6-phosphate | mutant F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 49 | - |
2-nitrophenyl beta-D-galactopyranoside-6-phosphate | wild-type, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 69 | - |
2-nitrophenyl beta-D-galactopyranoside-6-phosphate | mutant E417S/M424K/F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 171 | - |
2-nitrophenyl beta-D-galactopyranoside-6-phosphate | mutant E417S, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 175 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant E417S/M424K/F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 772 | - |
2-nitrophenyl beta-D-glucopyranoside | mutant M424K, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 777 | - |
2-nitrophenyl beta-D-glucopyranoside | mutant E417S/M424K/F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 840 | - |
2-nitrophenyl beta-D-glucopyranoside | mutant F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 860 | - |
2-nitrophenyl beta-D-glucopyranoside | wild-type, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 892 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 1060 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant E417S, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 1880 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant M424K, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 3300 | - |
2-nitrophenyl beta-D-glucopyranoside | mutant E417S, pH 5.0, 90°C | Pyrococcus furiosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 4 | - |
for 2-nitrophenyl-beta-1,4-D-galactopyranoside-6-phosphate | Pyrococcus furiosus |
3.2.1.21 | 5 | - |
for 2-nitrophenyl-beta-1,4-D-gluco- and galactopyranoside | Pyrococcus furiosus |
3.2.1.B28 | 4 | - |
wild-type, substrate 2-nitrophenyl beta-D-galactopyranoside-6-phosphate | Pyrococcus furiosus |
3.2.1.B28 | 5 | - |
mutant E417S/M424K/F426Y, substrates 2-nitrophenyl beta-D-glucopyranoside, 2-nitrophenyl beta-D-galactopyranoside | Pyrococcus furiosus |
3.2.1.B28 | 5 | - |
wild-type, substrates 2-nitrophenyl beta-D-glucopyranoside, 2-nitrophenyl beta-D-galactopyranoside | Pyrococcus furiosus |
3.2.1.B28 | 6 | - |
mutant E417S/M424K/F426Y, substrate 2-nitrophenyl beta-D-galactopyranoside-6-phosphate | Pyrococcus furiosus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B28 | 0.7 | - |
2-nitrophenyl beta-D-galactopyranoside-6-phosphate | mutant F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 1.6 | - |
2-nitrophenyl beta-D-galactopyranoside-6-phosphate | wild-type, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 4.2 | - |
2-nitrophenyl beta-D-galactopyranoside-6-phosphate | mutant E417S/M424K/F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 4.4 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant E417S/M424K/F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 7.7 | - |
2-nitrophenyl beta-D-galactopyranoside-6-phosphate | mutant E417S, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 13 | - |
2-nitrophenyl beta-D-glucopyranoside | mutant E417S/M424K/F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 25.3 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant E417S, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 113 | - |
2-nitrophenyl beta-D-glucopyranoside | mutant E417S, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 396 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant M424K, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 899 | - |
2-nitrophenyl beta-D-galactopyranoside | wild-type, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 1000 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 1484 | - |
2-nitrophenyl beta-D-glucopyranoside | mutant F426Y, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 1510 | - |
2-nitrophenyl beta-D-glucopyranoside | mutant M424K, pH 5.0, 90°C | Pyrococcus furiosus | |
3.2.1.B28 | 4048 | - |
2-nitrophenyl beta-D-glucopyranoside | wild-type, pH 5.0, 90°C | Pyrococcus furiosus |