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Literature summary extracted from

  • Yamamoto, M.; Horikoshi, K.
    Purification and properties of an oligo-1,6-glucosidase from alkalophilic Bacillus species (1990), Carbohydr. Res., 197, 227-235.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.10 Cu2+ complete inhibition by 1 mM CuCl2 Bacillus sp. F5
3.2.1.10 Fe2+ 90% inhibition by 1 mM FeCl2 Bacillus sp. F5
3.2.1.10 Hg2+ complete inhibition by 1 mM HgCl2 Bacillus sp. F5
3.2.1.10 monoiodoacetate 77% inhibition by 1 mM Bacillus sp. F5
3.2.1.10 p-chloromercuribenzoate complete inhibition by 0.1 mM Bacillus sp. F5
3.2.1.10 Pb2+ complete inhibition by 1 mM PbCl2 Bacillus sp. F5
3.2.1.10 Zn2+ 99% inhibition by 1 mM ZnCl2 Bacillus sp. F5

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.10 1
-
panose
-
Bacillus sp. F5
3.2.1.10 1
-
Panitol
-
Bacillus sp. F5
3.2.1.10 1.7
-
isomaltotriose
-
Bacillus sp. F5
3.2.1.10 1.9
-
maltotriose
-
Bacillus sp. F5
3.2.1.10 3.33
-
isomaltose
-
Bacillus sp. F5
3.2.1.10 4
-
maltotriitol
-
Bacillus sp. F5
3.2.1.10 13.3
-
maltose
-
Bacillus sp. F5

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.10 60000 62000 SDS-PAGE Bacillus sp. F5
3.2.1.10 63000
-
Sepharyl S-200 gel filtration Bacillus sp. F5

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.10 Bacillus sp. F5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.10
-
Bacillus sp. F5

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.10 34.2
-
purified enzyme, isomaltose as substrate Bacillus sp. F5

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.10 isomaltosaccharides + H2O n = 2-6 Bacillus sp. F5 D-glucose
-
?
3.2.1.10 isomaltose + H2O
-
Bacillus sp. F5 2 D-glucose
-
?
3.2.1.10 isomaltotriose + H2O
-
Bacillus sp. F5 3 alpha-D-glucose
-
?
3.2.1.10 maltose + H2O
-
Bacillus sp. F5 2 D-glucose
-
?
3.2.1.10 maltotriitol + H2O
-
Bacillus sp. F5 D-glucose + maltitol
-
?
3.2.1.10 maltotriose + H2O
-
Bacillus sp. F5 maltose + D-glucose
-
?
3.2.1.10 panitol
-
Bacillus sp. F5 glucitol + maltitol
-
?
3.2.1.10 panose + H2O
-
Bacillus sp. F5 D-glucose + maltose
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.10 45
-
-
Bacillus sp. F5

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.10 50 60 stable up to 50°C, no activity at 60°C Bacillus sp. F5

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.10 6 6.5 at 45°C, isomaltose as substrate Bacillus sp. F5

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.10 6 9 at 50°C, 10 min incubation Bacillus sp. F5