EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.10 | Cu2+ | complete inhibition by 1 mM CuCl2 | Bacillus sp. F5 | |
3.2.1.10 | Fe2+ | 90% inhibition by 1 mM FeCl2 | Bacillus sp. F5 | |
3.2.1.10 | Hg2+ | complete inhibition by 1 mM HgCl2 | Bacillus sp. F5 | |
3.2.1.10 | monoiodoacetate | 77% inhibition by 1 mM | Bacillus sp. F5 | |
3.2.1.10 | p-chloromercuribenzoate | complete inhibition by 0.1 mM | Bacillus sp. F5 | |
3.2.1.10 | Pb2+ | complete inhibition by 1 mM PbCl2 | Bacillus sp. F5 | |
3.2.1.10 | Zn2+ | 99% inhibition by 1 mM ZnCl2 | Bacillus sp. F5 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.10 | 1 | - |
panose | - |
Bacillus sp. F5 | |
3.2.1.10 | 1 | - |
Panitol | - |
Bacillus sp. F5 | |
3.2.1.10 | 1.7 | - |
isomaltotriose | - |
Bacillus sp. F5 | |
3.2.1.10 | 1.9 | - |
maltotriose | - |
Bacillus sp. F5 | |
3.2.1.10 | 3.33 | - |
isomaltose | - |
Bacillus sp. F5 | |
3.2.1.10 | 4 | - |
maltotriitol | - |
Bacillus sp. F5 | |
3.2.1.10 | 13.3 | - |
maltose | - |
Bacillus sp. F5 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.10 | 60000 | 62000 | SDS-PAGE | Bacillus sp. F5 |
3.2.1.10 | 63000 | - |
Sepharyl S-200 gel filtration | Bacillus sp. F5 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.10 | Bacillus sp. F5 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.10 | - |
Bacillus sp. F5 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.10 | 34.2 | - |
purified enzyme, isomaltose as substrate | Bacillus sp. F5 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.10 | isomaltosaccharides + H2O | n = 2-6 | Bacillus sp. F5 | D-glucose | - |
? | |
3.2.1.10 | isomaltose + H2O | - |
Bacillus sp. F5 | 2 D-glucose | - |
? | |
3.2.1.10 | isomaltotriose + H2O | - |
Bacillus sp. F5 | 3 alpha-D-glucose | - |
? | |
3.2.1.10 | maltose + H2O | - |
Bacillus sp. F5 | 2 D-glucose | - |
? | |
3.2.1.10 | maltotriitol + H2O | - |
Bacillus sp. F5 | D-glucose + maltitol | - |
? | |
3.2.1.10 | maltotriose + H2O | - |
Bacillus sp. F5 | maltose + D-glucose | - |
? | |
3.2.1.10 | panitol | - |
Bacillus sp. F5 | glucitol + maltitol | - |
? | |
3.2.1.10 | panose + H2O | - |
Bacillus sp. F5 | D-glucose + maltose | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.10 | 45 | - |
- |
Bacillus sp. F5 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.10 | 50 | 60 | stable up to 50°C, no activity at 60°C | Bacillus sp. F5 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.10 | 6 | 6.5 | at 45°C, isomaltose as substrate | Bacillus sp. F5 |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.10 | 6 | 9 | at 50°C, 10 min incubation | Bacillus sp. F5 |