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Literature summary extracted from

  • Suzuki, Y.; Aoki, R.; Hayashi, H.
    Assignment of a p-nitrophenyl-alpha-D-glucopyranoside hydrolyzing alpha-glucosidase of Bacillus cereus ATCC 7064 to an exo-oligo-1,6-glucosidase (1982), Biochim. Biophys. Acta, 704, 476-483.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.10 Ba2+
-
Bacillus cereus
3.2.1.10 Co2+ 96% inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate Bacillus cereus
3.2.1.10 Cu2+ 95% inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate Bacillus cereus
3.2.1.10 D-glucose
-
Bacillus cereus
3.2.1.10 EDTA no inhibition by 2 mM at pH 7.5 Bacillus cereus
3.2.1.10 Fe2+ complete inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate Bacillus cereus
3.2.1.10 glucono-delta-lactone
-
Bacillus cereus
3.2.1.10 Hg2+ complete inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate Bacillus cereus
3.2.1.10 Mn2+ 40% inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate Bacillus cereus
3.2.1.10 p-chloromercuribenzoate no inhibition by 2 mM with p-nitrophenyl-alpha-D-glucopyranoside as substrate Bacillus cereus
3.2.1.10 p-nitrophenol
-
Bacillus cereus
3.2.1.10 Pb2+ 90% inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate Bacillus cereus
3.2.1.10 phenyl alpha-D-glucoside
-
Bacillus cereus
3.2.1.10 Tris 93% inhibition by 5 mM Bacillus cereus
3.2.1.10 Urea 50% inactivation after treatment with 1.42 M at 25°C or 0.74 M at 35°C for 5 h Bacillus cereus
3.2.1.10 Zn2+ 96% inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.10 0.81
-
p-nitrophenyl-alpha-D-glucopyranoside
-
Bacillus cereus
3.2.1.10 2.2
-
phenyl-alpha-D-glucopyranoside
-
Bacillus cereus
3.2.1.10 2.8
-
isomaltotetraose
-
Bacillus cereus
3.2.1.10 3
-
isomaltotriose
-
Bacillus cereus
3.2.1.10 4.6
-
isomaltopentaose
-
Bacillus cereus
3.2.1.10 6.2
-
isomaltose
-
Bacillus cereus
3.2.1.10 11
-
isomaltohexaose
-
Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.10 60000 62000 SDS-PAGE Bacillus cereus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.10 Ethanol fairly stable for 5 h at 4°C in 20% ethanol , 50% inactivation after 5 h treatment with 17% ethanol at 25°C and pH 6.8 Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.10 Bacillus cereus
-
ATCC 7064
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.10
-
Bacillus cereus

Storage Stability

EC Number Storage Stability Organism
3.2.1.10 -20°C, purified enzyme, 6 months, no loss of activity Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.10 alpha-limit dextrins + H2O
-
Bacillus cereus D-glucose
-
?
3.2.1.10 isomaltohexaose + H2O
-
Bacillus cereus 6 alpha-D-glucose
-
?
3.2.1.10 isomaltopentaose + H2O
-
Bacillus cereus 5 alpha-D-glucose
-
?
3.2.1.10 isomaltosaccharides + H2O n = 2-6 Bacillus cereus D-glucose
-
?
3.2.1.10 isomaltose + H2O
-
Bacillus cereus 2 D-glucose
-
?
3.2.1.10 isomaltotetraose + H2O
-
Bacillus cereus 4 alpha-D-glucose
-
?
3.2.1.10 isomaltotriose + H2O
-
Bacillus cereus 3 alpha-D-glucose
-
?
3.2.1.10 p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
Bacillus cereus D-glucose + p-nitrophenol
-
?
3.2.1.10 panose + H2O
-
Bacillus cereus D-glucose + maltose
-
?
3.2.1.10 phenyl-alpha-D-glucopyranoside + H2O
-
Bacillus cereus D-glucose + phenol
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.10 alpha-glucosidase
-
Bacillus cereus
3.2.1.10 dextrin 6-alpha-D-glucanohydrolase
-
Bacillus cereus
3.2.1.10 exo-oligo-1,6-glucosidase
-
Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.10 41
-
-
Bacillus cereus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.10 27 53 half-optima at 27°C and 53°C, no activity at 60°C Bacillus cereus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.10 13 55 heating for 10 min in 33.3 mM phosphate buffer, pH 6.8: no loss of activity from 13 to 40°C, 87% of activity at 45°C, 56% at 48°C, 25% at 50°C, no activity at 55°C Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.10 6.7
-
-
Bacillus cereus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.10 4.9 9.5 half-optima at pH 5.5 and 8.2 Bacillus cereus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.10 5.7 9 stable at 25°C for 15 h Bacillus cereus