EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.10 | Ba2+ | - |
Bacillus cereus | |
3.2.1.10 | Co2+ | 96% inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus | |
3.2.1.10 | Cu2+ | 95% inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus | |
3.2.1.10 | D-glucose | - |
Bacillus cereus | |
3.2.1.10 | EDTA | no inhibition by 2 mM at pH 7.5 | Bacillus cereus | |
3.2.1.10 | Fe2+ | complete inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus | |
3.2.1.10 | glucono-delta-lactone | - |
Bacillus cereus | |
3.2.1.10 | Hg2+ | complete inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus | |
3.2.1.10 | Mn2+ | 40% inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus | |
3.2.1.10 | p-chloromercuribenzoate | no inhibition by 2 mM with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus | |
3.2.1.10 | p-nitrophenol | - |
Bacillus cereus | |
3.2.1.10 | Pb2+ | 90% inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus | |
3.2.1.10 | phenyl alpha-D-glucoside | - |
Bacillus cereus | |
3.2.1.10 | Tris | 93% inhibition by 5 mM | Bacillus cereus | |
3.2.1.10 | Urea | 50% inactivation after treatment with 1.42 M at 25°C or 0.74 M at 35°C for 5 h | Bacillus cereus | |
3.2.1.10 | Zn2+ | 96% inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.10 | 0.81 | - |
p-nitrophenyl-alpha-D-glucopyranoside | - |
Bacillus cereus | |
3.2.1.10 | 2.2 | - |
phenyl-alpha-D-glucopyranoside | - |
Bacillus cereus | |
3.2.1.10 | 2.8 | - |
isomaltotetraose | - |
Bacillus cereus | |
3.2.1.10 | 3 | - |
isomaltotriose | - |
Bacillus cereus | |
3.2.1.10 | 4.6 | - |
isomaltopentaose | - |
Bacillus cereus | |
3.2.1.10 | 6.2 | - |
isomaltose | - |
Bacillus cereus | |
3.2.1.10 | 11 | - |
isomaltohexaose | - |
Bacillus cereus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.10 | 60000 | 62000 | SDS-PAGE | Bacillus cereus |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.2.1.10 | Ethanol | fairly stable for 5 h at 4°C in 20% ethanol , 50% inactivation after 5 h treatment with 17% ethanol at 25°C and pH 6.8 | Bacillus cereus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.10 | Bacillus cereus | - |
ATCC 7064 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.10 | - |
Bacillus cereus |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.10 | -20°C, purified enzyme, 6 months, no loss of activity | Bacillus cereus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.10 | alpha-limit dextrins + H2O | - |
Bacillus cereus | D-glucose | - |
? | |
3.2.1.10 | isomaltohexaose + H2O | - |
Bacillus cereus | 6 alpha-D-glucose | - |
? | |
3.2.1.10 | isomaltopentaose + H2O | - |
Bacillus cereus | 5 alpha-D-glucose | - |
? | |
3.2.1.10 | isomaltosaccharides + H2O | n = 2-6 | Bacillus cereus | D-glucose | - |
? | |
3.2.1.10 | isomaltose + H2O | - |
Bacillus cereus | 2 D-glucose | - |
? | |
3.2.1.10 | isomaltotetraose + H2O | - |
Bacillus cereus | 4 alpha-D-glucose | - |
? | |
3.2.1.10 | isomaltotriose + H2O | - |
Bacillus cereus | 3 alpha-D-glucose | - |
? | |
3.2.1.10 | p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Bacillus cereus | D-glucose + p-nitrophenol | - |
? | |
3.2.1.10 | panose + H2O | - |
Bacillus cereus | D-glucose + maltose | - |
? | |
3.2.1.10 | phenyl-alpha-D-glucopyranoside + H2O | - |
Bacillus cereus | D-glucose + phenol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.10 | alpha-glucosidase | - |
Bacillus cereus |
3.2.1.10 | dextrin 6-alpha-D-glucanohydrolase | - |
Bacillus cereus |
3.2.1.10 | exo-oligo-1,6-glucosidase | - |
Bacillus cereus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.10 | 41 | - |
- |
Bacillus cereus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.10 | 27 | 53 | half-optima at 27°C and 53°C, no activity at 60°C | Bacillus cereus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.10 | 13 | 55 | heating for 10 min in 33.3 mM phosphate buffer, pH 6.8: no loss of activity from 13 to 40°C, 87% of activity at 45°C, 56% at 48°C, 25% at 50°C, no activity at 55°C | Bacillus cereus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.10 | 6.7 | - |
- |
Bacillus cereus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.10 | 4.9 | 9.5 | half-optima at pH 5.5 and 8.2 | Bacillus cereus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.10 | 5.7 | 9 | stable at 25°C for 15 h | Bacillus cereus |