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Literature summary extracted from

  • Suzuki, Y.; Tomura, Y.
    Purification and characterization of Bacillus coagulans oligo-1,6-glucosidase (1986), Eur. J. Biochem., 158, 77-83.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.10 Ba2+
-
 Bacillus cereus
3.2.1.10 Ba2+ 72% inhibition by 2 mM BaCl2 Weizmannia coagulans
3.2.1.10 Ca2+ 29% inhibition by 2 mM CaCl2 Weizmannia coagulans
3.2.1.10 Cd2+ complete inhibition Weizmannia coagulans
3.2.1.10 Co2+
-
 Bacillus cereus
3.2.1.10 Co2+ complete inhibition Weizmannia coagulans
3.2.1.10 Cu2+
-
 Bacillus cereus
3.2.1.10 Cu2+ 96% inhibition by 2 mM CuCl2 Weizmannia coagulans
3.2.1.10 D-glucose
-
 Bacillus cereus
3.2.1.10 D-glucose 52% inhibition by 10 mM, competitive Weizmannia coagulans
3.2.1.10 EDTA no inhibition by 2 mM at pH 7.5 Bacillus cereus
3.2.1.10 EDTA no inhibition by 2 mM at pH 7.5 Weizmannia coagulans
3.2.1.10 Fe3+ 86% inhibition by 2 mM FeCl3 Weizmannia coagulans
3.2.1.10 glucono-delta-lactone
-
 Bacillus cereus
3.2.1.10 glucono-delta-lactone 57% inhibition by 10 mM, competitive Weizmannia coagulans
3.2.1.10 Hg2+
-
 Bacillus cereus
3.2.1.10 Hg2+ 99% inhibition by 2 mM HgCl2 Weizmannia coagulans
3.2.1.10 Mg2+ 42% inhibition by 2 mM MgCl2 Weizmannia coagulans
3.2.1.10 Mn2+ 92% inhibition by 2 mM MbCl2 Weizmannia coagulans
3.2.1.10 Ni2+ 94% inhibition by 2 mM NiCl2 Weizmannia coagulans
3.2.1.10 p-nitrophenol
-
 Bacillus cereus
3.2.1.10 p-nitrophenol complete inhibition by 2 mM Weizmannia coagulans
3.2.1.10 Pb2+
-
 Bacillus cereus
3.2.1.10 Pb2+ complete inhibition Weizmannia coagulans
3.2.1.10 phenol in combination with Tris most potent inhibitor Weizmannia coagulans
3.2.1.10 phenyl alpha-D-glucoside
-
 Bacillus cereus
3.2.1.10 phenyl alpha-D-glucoside 58% inhibition by 10 mM Weizmannia coagulans
3.2.1.10 Phenyl alpha-maltoside 92% inhibition by 10 mM, noncompetitive Weizmannia coagulans
3.2.1.10 Sn2+ complete inhibition Weizmannia coagulans
3.2.1.10 Tris
-
 Bacillus cereus
3.2.1.10 Tris 89% inhibition by 5 mM, competitive Weizmannia coagulans
3.2.1.10 Urea
-
 Bacillus cereus
3.2.1.10 Urea 50% inactivation after treatment with 4.6 M for 5 h at 25°C and pH 6.8 Weizmannia coagulans
3.2.1.10 Zn2+
-
 Bacillus cereus
3.2.1.10 Zn2+ complete inhibition Weizmannia coagulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.10 0.17
-
 p-nitrophenyl alpha-D-glucopyranoside
-
 Weizmannia coagulans
3.2.1.10 3
-
 isomaltose
-
 Weizmannia coagulans
3.2.1.10 4.4
-
 isomaltotriose
-
 Weizmannia coagulans
3.2.1.10 7.4
-
 isomaltotetraose
-
 Weizmannia coagulans
3.2.1.10 13
-
 isomaltopentaose
-
 Weizmannia coagulans
3.2.1.10 15
-
 isomaltohexaose
-
 Weizmannia coagulans

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.10 Ethanol
-
 Bacillus cereus
3.2.1.10 Ethanol 50% inactivation after 5 h treatment with 32% ethanol at 25°C and pH 6.8 Weizmannia coagulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.10 Bacillus cereus
-
 ATCC 7064
-
3.2.1.10 Weizmannia coagulans
-
 ATCC 7050
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.10
-
 Weizmannia coagulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.10 alpha-limit dextrins + H2O
-
 Bacillus cereus D-glucose
-
 ?
3.2.1.10 alpha-limit dextrins + H2O
-
 Weizmannia coagulans D-glucose
-
 ?
3.2.1.10 isomaltohexaose + H2O
-
 Weizmannia coagulans 6 alpha-D-glucose
-
 ?
3.2.1.10 isomaltopentaose + H2O
-
 Weizmannia coagulans 5 alpha-D-glucose
-
 ?
3.2.1.10 isomaltosaccharides + H2O n = 2-6 Bacillus cereus D-glucose
-
 ?
3.2.1.10 isomaltosaccharides + H2O n = 2-6 Weizmannia coagulans D-glucose
-
 ?
3.2.1.10 isomaltose + H2O
-
 Weizmannia coagulans alpha-D-glucose + D-glucose
-
 ?
3.2.1.10 isomaltotetraose + H2O
-
 Weizmannia coagulans 4 alpha-D-glucose
-
 ?
3.2.1.10 isomaltotriose + H2O
-
 Weizmannia coagulans 3 alpha-D-glucose
-
 ?
3.2.1.10 p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
 Bacillus cereus D-glucose + p-nitrophenol
-
 ?
3.2.1.10 p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
 Weizmannia coagulans D-glucose + p-nitrophenol
-
 ?
3.2.1.10 panose + H2O
-
 Bacillus cereus D-glucose + maltose
-
 ?
3.2.1.10 panose + H2O
-
 Weizmannia coagulans D-glucose + maltose
-
 ?
3.2.1.10 phenyl-alpha-D-glucopyranoside + H2O
-
 Weizmannia coagulans D-glucose + phenol
-
 ?
3.2.1.10 sucrose + H2O
-
 Weizmannia coagulans D-glucose + D-fructose
-
 ?
3.2.1.10 turanose + H2O
-
 Weizmannia coagulans D-glucose + D-fructose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.10 62
-
-
 Weizmannia coagulans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.10 47 75 half-optima at 47°C and 75°C, no activity at 78°C Weizmannia coagulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.10 53
-
 stable at pH 6.8 for 10 min Weizmannia coagulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.10 5.2
-
-
 Weizmannia coagulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.10 4.1 10.2 half-optima at pH 4.8 and 7.3 Weizmannia coagulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.10 5 9.1 50% inactivation at pH 5.0 and 9.1 after incubation at 34°C for 15 h Weizmannia coagulans
3.2.1.10 6.5 7.3 50% inactivation at pH 6.5 and 7.3 after incubation at 34°C for 15 h Bacillus cereus