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Literature summary extracted from
- Cloning and expression in Escherichia coli of an extremely thermostable oligo-1,6-glucosidase gene from Bacillus thermoglucosidasius (1989), J. Bacteriol., 171, 1219-1222.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.10 | expression in Escherichia coli | Parageobacillus thermoglucosidasius |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.10 | Parageobacillus thermoglucosidasius | - |
KP 1006 | - |
| 3.2.1.10 | Parageobacillus thermoglucosidasius KP 1006 | - |
KP 1006 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.10 | after expression in Escherichia coli | Parageobacillus thermoglucosidasius |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.10 | 313 | - |
purified enzyme after expression in Escherichia coli, p-nitrophenyl-alpha-D-glucopyranoside as substrate | Parageobacillus thermoglucosidasius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.10 | isomaltosaccharides + H2O | - |
Parageobacillus thermoglucosidasius | D-glucose | - |
? |  |
| 3.2.1.10 | isomaltosaccharides + H2O | - |
Parageobacillus thermoglucosidasius KP 1006 | D-glucose | - |
? | |
| 3.2.1.10 | isomaltose + H2O | - |
Parageobacillus thermoglucosidasius | 2 D-glucose | - |
? | |
| 3.2.1.10 | isomaltose + H2O | - |
Parageobacillus thermoglucosidasius KP 1006 | 2 D-glucose | - |
? | |
| 3.2.1.10 | additional information | no maltase activity | Parageobacillus thermoglucosidasius | ? | - |
? | |
| 3.2.1.10 | additional information | no maltase activity | Parageobacillus thermoglucosidasius KP 1006 | ? | - |
? | |
| 3.2.1.10 | p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Parageobacillus thermoglucosidasius | D-glucose + p-nitrophenol | - |
? | |
| 3.2.1.10 | p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Parageobacillus thermoglucosidasius KP 1006 | D-glucose + p-nitrophenol | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.10 | dextrin 6-alpha-D-glucanohydrolase | - |
Parageobacillus thermoglucosidasius |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.10 | 70 | 75 | - |
Parageobacillus thermoglucosidasius |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.10 | 30 | 85 | stable from 30 to 70°C, 50% inactivation at 71°C after 10 min | Parageobacillus thermoglucosidasius |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.10 | 6.8 | - |
- |
Parageobacillus thermoglucosidasius |
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Release 2023.1 (January 2023)
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