EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.7 | HgCl2 | inhibition partially reversed by DTT, 2-mercaptoethanol, or reduced glutathione | Rhodococcus rhodochrous | |
3.5.5.7 | NH3 | inhibition partially reversed by DTT, 2-mercaptoethanol, or reduced glutathione | Rhodococcus rhodochrous | |
3.5.5.7 | PCMB | - |
Rhodococcus rhodochrous |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.5.7 | 1.14 | - |
acrylonitrile | - |
Rhodococcus rhodochrous | |
3.5.5.7 | 18.9 | - |
Crotonitrile | - |
Rhodococcus rhodochrous |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.7 | additional information | enzyme contains no metal | Rhodococcus rhodochrous |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.5.7 | 41000 | - |
or hexadecamer, 15 * or 16 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol | Rhodococcus rhodochrous |
3.5.5.7 | 41000 | - |
or pentadecamer, 16 * or 15 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol | Rhodococcus rhodochrous |
3.5.5.7 | 604000 | - |
equilibrium sedimentation | Rhodococcus rhodochrous |
3.5.5.7 | 650000 | - |
HPLC gel filtration | Rhodococcus rhodochrous |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.5.7 | Rhodococcus rhodochrous | - |
- |
- |
3.5.5.7 | Rhodococcus rhodochrous K22 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.5.7 | - |
Rhodococcus rhodochrous |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.5.7 | 0.737 | - |
- |
Rhodococcus rhodochrous |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.5.7 | 1,4-dicyano-2-butene + H2O | 42.6% of the activity with crotononitrile | Rhodococcus rhodochrous | ? | - |
? | |
3.5.5.7 | 1-cyclohexeneacetonitrile + H2O | 18.2% of the activity with crotononitrile | Rhodococcus rhodochrous | 1-cyclohexene carboxylate + NH3 | - |
? | |
3.5.5.7 | 1-cyclopenteneacetonitrile + H2O | 24.9% of the activity with crotononitrile | Rhodococcus rhodochrous | 1-cyclopentene carboxylate + NH3 | - |
? | |
3.5.5.7 | 2,4-dicyano-1-butene + H2O | 127% of the activity with crotononitrile | Rhodococcus rhodochrous | ? | - |
? | |
3.5.5.7 | 2-aminocrotononitrile + H2O | 30.3% of the activity with crotononitrile | Rhodococcus rhodochrous | 2-aminocrotonic acid + NH3 | - |
? | |
3.5.5.7 | 2-aminocrotononitrile + H2O | 30.3% of the activity with crotononitrile | Rhodococcus rhodochrous K22 | 2-aminocrotonic acid + NH3 | - |
? | |
3.5.5.7 | 2-chloroacrylonitrile + H2O | 47.1% of the activity with crotononitrile | Rhodococcus rhodochrous | 2-chloroacrylic acid + NH3 | - |
? | |
3.5.5.7 | 2-furonitrile + H2O | 52.0% of the activity with crotononitrile | Rhodococcus rhodochrous | furoic acid + NH3 | - |
? | |
3.5.5.7 | 2-methyl-2-butenenitrile + H2O | 14.9% of the activity with crotononitrile | Rhodococcus rhodochrous | 2-methyl-2-butenoic acid + NH3 | - |
? | |
3.5.5.7 | 2-methyl-3-butenenitrile + H2O | 46.8% of the activity with crotononitrile | Rhodococcus rhodochrous | 2-methyl-3-butenoic acid + NH3 | - |
? | |
3.5.5.7 | 2-pentenenitrile + H2O | 6.33% of the activity with crotononitrile | Rhodococcus rhodochrous | 2-pentenoic acid + NH3 | - |
? | |
3.5.5.7 | 2-thiopheneacetonitrile + H2O | 73.5% of the activity with crotononitrile | Rhodococcus rhodochrous | thiophene-2-carboxylate + NH3 | - |
? | |
3.5.5.7 | 2-thiophenecarbonitrile + H2O | 61.1% of the activity with crotononitrile | Rhodococcus rhodochrous | 2-thiophenecarboxylate + NH3 | - |
? | |
3.5.5.7 | 3,3'-oxydipropionitrile + H2O | 29.0% of the activity with crotononitrile | Rhodococcus rhodochrous | ? + NH3 | - |
? | |
3.5.5.7 | 3-chloropropionitrile + H2O | 113% of the activity with crotononitrile | Rhodococcus rhodochrous | 3-chloropropanoate + NH3 | - |
? | |
3.5.5.7 | 3-cyanopyridine + H2O | 7.72% of the activity with crotononitrile | Rhodococcus rhodochrous | 3-carboxypyridine + NH3 | - |
? | |
3.5.5.7 | 3-ethoxyacrylonitrile + H2O | 19.1% of the activity with crotononitrile | Rhodococcus rhodochrous | 3-ethoxyacrylic acid + NH3 | - |
? | |
3.5.5.7 | 3-ethoxyacrylonitrile + H2O | 19.1% of the activity with crotononitrile | Rhodococcus rhodochrous K22 | 3-ethoxyacrylic acid + NH3 | - |
? | |
3.5.5.7 | 3-ethoxybenzonitrile + H2O | 21.1% of the activity with crotononitrile | Rhodococcus rhodochrous | 3-ethoxybenzoate + NH3 | - |
? | |
3.5.5.7 | 3-methoxybenzonitrile | 24.8% of the activity with crotononitrile | Rhodococcus rhodochrous | 3-methoxybenzoate + NH3 | - |
? | |
3.5.5.7 | 3-nitrobenzonitrile + H2O | 74.5% of the activity with crotononitrile | Rhodococcus rhodochrous | 3-nitrobenzoate + NH3 | - |
? | |
3.5.5.7 | 3-pentenenitrile + H2O | 132% of the activity with crotononitrile | Rhodococcus rhodochrous | 3-pentenoic acid + NH3 | - |
? | |
3.5.5.7 | 3-thiopheneacetonitrile + H2O | 66.3% of the activity with crotononitrile | Rhodococcus rhodochrous | thiophene-3-carboxylate + NH3 | - |
? | |
3.5.5.7 | 3-tolunitrile + H2O | 67.9% of the activity with crotononitrile | Rhodococcus rhodochrous | 3-methylbenzenecarboxylic acid + NH3 | - |
? | |
3.5.5.7 | 3-xylylene dicyanide + H2O | 14.62% of the activity with crotononitrile | Rhodococcus rhodochrous | ? | - |
? | |
3.5.5.7 | 4-chlorobutyronitrile + H2O | 116% of the activity with crotononitrile | Rhodococcus rhodochrous | 4-chlorobutyrate + NH3 | - |
? | |
3.5.5.7 | 4-cyano-1-cyclohexene + H2O | 7.03% of the activity with crotononitrile | Rhodococcus rhodochrous | 1-cyclohexene-4-carboxylate + NH3 | - |
? | |
3.5.5.7 | 4-cyanopyridine + H2O | 9.71% of the activity with crotononitrile | Rhodococcus rhodochrous | 4-carboxypyridine + NH3 | - |
? | |
3.5.5.7 | acetonitrile + H2O | 28.3% of the activity with crotononitrile | Rhodococcus rhodochrous | acetate + NH3 | - |
? | |
3.5.5.7 | acrylonitrile + H2O | 348% of the activity with crotononitrile | Rhodococcus rhodochrous | acrylic acid + NH3 | - |
? | |
3.5.5.7 | acrylonitrile + H2O | 348% of the activity with crotononitrile | Rhodococcus rhodochrous K22 | acrylic acid + NH3 | - |
? | |
3.5.5.7 | adiponitrile + H2O | 110% of the activity with crotononitrile | Rhodococcus rhodochrous | adipic acid + NH3 | - |
? | |
3.5.5.7 | benzonitrile + 2 H2O | 27.1% of the activity with crotononitrile | Rhodococcus rhodochrous | benzoate + NH3 | - |
? | |
3.5.5.7 | butyronitrile + H2O | 18.0% of the activity with crotononitrile | Rhodococcus rhodochrous | butyrate + NH3 | - |
? | |
3.5.5.7 | capronitrile + H2O | 38.7% of the activity with crotononitrile | Rhodococcus rhodochrous | hexanoate + NH3 | - |
? | |
3.5.5.7 | chloroacetonitrile + H2O | 61.7% of the activity with crotononitrile | Rhodococcus rhodochrous | chloroacetate + NH3 | - |
? | |
3.5.5.7 | cinnamonitrile + H2O | 31.2% of the activity with crotononitrile | Rhodococcus rhodochrous | cinnamate + NH3 | - |
? | |
3.5.5.7 | crotonitrile + H2O | - |
Rhodococcus rhodochrous | crotonic acid + NH3 | - |
? | |
3.5.5.7 | crotonitrile + H2O | - |
Rhodococcus rhodochrous K22 | crotonic acid + NH3 | - |
? | |
3.5.5.7 | cyanoacetic acid ethyl ester + H2O | 117% of the activity withcrotononitrile | Rhodococcus rhodochrous | acetic acid ethyl ester + NH3 | - |
? | |
3.5.5.7 | cyanopyrazine + H2O | 17.3% of the activity with crotononitrile | Rhodococcus rhodochrous | pyrazine-2-carboxylate + NH3 | - |
? | |
3.5.5.7 | cyclopentanecarbonitrile + H2O | 11.7% of the activity with crotononitrile | Rhodococcus rhodochrous | cyclopentanoic acid + NH3 | - |
? | |
3.5.5.7 | cyclopropanecarbonitrile + H2O | 22.9% of the activity with crotononitrile | Rhodococcus rhodochrous | cyclopropanoic acid + NH3 | - |
? | |
3.5.5.7 | diaminomaleonitrile + H2O | 18.6% of the activity with crotononitrile | Rhodococcus rhodochrous | diaminomaleic acid + NH3 | - |
? | |
3.5.5.7 | fumaronitrile + H2O | 27.4% of the activity with crotononitrile | Rhodococcus rhodochrous | fumaric acid + NH3 | - |
? | |
3.5.5.7 | glutaronitrile + H2O | 345% of the activity with crotononitrile | Rhodococcus rhodochrous | glutarate + NH3 | - |
? | |
3.5.5.7 | iminodiacetonitrile + 2 H2O | 261% of the activity with crotononitrile | Rhodococcus rhodochrous | iminodiacetic acid + 2 NH3 | - |
? | |
3.5.5.7 | isobutyronitrile + H2O | 6.74% of the activity with crotononitrile | Rhodococcus rhodochrous | isobutyrate + NH3 | - |
? | |
3.5.5.7 | isocapronitrile + H2O | 41.5% of the activity with crotononitrile | Rhodococcus rhodochrous | isohexanoate + NH3 | - |
? | |
3.5.5.7 | isophthalonitrile + H2O | 66.1% of the activity with crotononitrile | Rhodococcus rhodochrous | isophthalate + NH3 | - |
? | |
3.5.5.7 | malononitrile + H2O | 45.1% of the activity with crotononitrile | Rhodococcus rhodochrous | malonic acid + NH3 | - |
? | |
3.5.5.7 | methacrylonitrile + H2O | 143% of the activity with crotononitrile | Rhodococcus rhodochrous | methacrylic acid + NH3 | - |
? | |
3.5.5.7 | methacrylonitrile + H2O | 143% of the activity with crotononitrile | Rhodococcus rhodochrous K22 | methacrylic acid + NH3 | - |
? | |
3.5.5.7 | methoxyacetonitrile + H2O | 37.4% of the activity with crotononitrile | Rhodococcus rhodochrous | methoxyacetate + NH3 | - |
? | |
3.5.5.7 | N-methyl-beta-alaninenitrile + H2O | 11.4% of the activity with crotononitrile | Rhodococcus rhodochrous | N-methyl-beta-Ala + NH3 | - |
? | |
3.5.5.7 | phenylacetonitrile + 2 H2O | 27.3% of the activity with crotononitrile | Rhodococcus rhodochrous | phenylacetate + NH3 | - |
? | |
3.5.5.7 | pimelonitrile + H2O | 27.3% of the activity with crotononitrile | Rhodococcus rhodochrous | pimelic acid + NH3 | - |
? | |
3.5.5.7 | piperonylonitrile + H2O | 6.8% of the activity with crotononitrile | Rhodococcus rhodochrous | (3,4-methylenedioxy)benzoate + NH3 | - |
? | |
3.5.5.7 | propionitrile + H2O | 12.7% of the activity with crotononitrile | Rhodococcus rhodochrous | propionate + NH3 | - |
? | |
3.5.5.7 | sebaconitrile + H2O | 15.7% of the activity with crotononitrile | Rhodococcus rhodochrous | ? + NH3 | - |
? | |
3.5.5.7 | suberonitrile + H2O | 21.4% of the activity with crotononitrile | Rhodococcus rhodochrous | decanedioic acid + NH3 | - |
? | |
3.5.5.7 | succinonitrile + H2O | 271% of the activity with crotononitrile | Rhodococcus rhodochrous | succinate + NH3 | - |
? | |
3.5.5.7 | valeronitrile + H2O | 40.8% of the activity with crotononitrile | Rhodococcus rhodochrous | pentanoate + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.5.7 | hexadecamer | or pentadecamer, 16 * or 15 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol | Rhodococcus rhodochrous |
3.5.5.7 | pentadecamer | or hexadecamer, 15 * or 16 * 41000, SDS-PAGE in presence of 50 mM 2-mercaptoethanol | Rhodococcus rhodochrous |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.7 | 50 | - |
- |
Rhodococcus rhodochrous |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.7 | 40 | 55 | 40°C: about 40% of maximal activity, 55°C: about 60% of maximal activity, crotonitrile | Rhodococcus rhodochrous |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.7 | 25 | - |
pH 7.5, 1 h, stable | Rhodococcus rhodochrous |
3.5.5.7 | 30 | - |
pH 7.5, 1 h, 6% loss of activity | Rhodococcus rhodochrous |
3.5.5.7 | 35 | - |
pH 7.5, 1 h, 8% loss of activity | Rhodococcus rhodochrous |
3.5.5.7 | 40 | - |
pH 7.5, 1 h, 13% loss of activity | Rhodococcus rhodochrous |
3.5.5.7 | 45 | - |
pH 7.5, 1 h, 72% loss of activity | Rhodococcus rhodochrous |
3.5.5.7 | 50 | - |
pH 7.5, 1 h, 93% loss of activity | Rhodococcus rhodochrous |
3.5.5.7 | 55 | - |
pH 7.5, 1 h, 100% loss of activity | Rhodococcus rhodochrous |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.7 | 5.5 | - |
- |
Rhodococcus rhodochrous |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.7 | 6 | 8 | 25°C, 45 min, less than 30% loss of activity | Rhodococcus rhodochrous |
3.5.5.7 | 10 | - |
25°C, 45 min, 40% loss of activity | Rhodococcus rhodochrous |