BRENDA - Enzyme Database

Purification of all glycolytic enzymes from one muscle extract

Scopes, R.K.; Stoter, A.; Methods Enzymol. 90, 479-490 (1982)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.33
cytoplasm
-
Oryctolagus cuniculus
5737
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.12
Oryctolagus cuniculus
-
-
-
3.2.1.33
Oryctolagus cuniculus
-
-
-
4.2.1.11
Oryctolagus cuniculus
-
-
-
5.4.2.2
Oryctolagus cuniculus
-
rabbit
-
5.4.2.11
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.33
-
Oryctolagus cuniculus
5.4.2.11
-
Oryctolagus cuniculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.12
muscle
of back and hind legs
Oryctolagus cuniculus
-
3.2.1.33
muscle
-
Oryctolagus cuniculus
-
4.2.1.11
heart
back and hind legs
Oryctolagus cuniculus
-
5.4.2.2
muscle
-
Oryctolagus cuniculus
-
5.4.2.11
muscle
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.33
3
-
-
Oryctolagus cuniculus
Storage Stability
EC Number
Storage Stability
Organism
3.2.1.33
5°C, crystalline suspension, ammonium sulfate, retains activity for many months or years
Oryctolagus cuniculus
3.2.1.33
frozen, concentrated solution without salt, retains activity for many months or years
Oryctolagus cuniculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.12
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
3247
Oryctolagus cuniculus
3-phospho-D-glyceroyl phosphate + NADH
-
3247
Oryctolagus cuniculus
?
4.2.1.11
2-phospho-D-glycerate
-
3247
Oryctolagus cuniculus
phosphoenolpyruvate
-
3247
Oryctolagus cuniculus
-
5.4.2.11
2-Phosphoglycerate
-
3247
Oryctolagus cuniculus
3-Phosphoglycerate
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.12
NAD+
-
Oryctolagus cuniculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.12
NAD+
-
Oryctolagus cuniculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.33
cytoplasm
-
Oryctolagus cuniculus
5737
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.33
-
Oryctolagus cuniculus
5.4.2.11
-
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.12
muscle
of back and hind legs
Oryctolagus cuniculus
-
3.2.1.33
muscle
-
Oryctolagus cuniculus
-
4.2.1.11
heart
back and hind legs
Oryctolagus cuniculus
-
5.4.2.2
muscle
-
Oryctolagus cuniculus
-
5.4.2.11
muscle
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.33
3
-
-
Oryctolagus cuniculus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.1.33
5°C, crystalline suspension, ammonium sulfate, retains activity for many months or years
Oryctolagus cuniculus
3.2.1.33
frozen, concentrated solution without salt, retains activity for many months or years
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.12
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
3247
Oryctolagus cuniculus
3-phospho-D-glyceroyl phosphate + NADH
-
3247
Oryctolagus cuniculus
?
4.2.1.11
2-phospho-D-glycerate
-
3247
Oryctolagus cuniculus
phosphoenolpyruvate
-
3247
Oryctolagus cuniculus
-
5.4.2.11
2-Phosphoglycerate
-
3247
Oryctolagus cuniculus
3-Phosphoglycerate
-
-
-
-