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Literature summary extracted from
- Development of a mutagenesis, expression and purification system for yeast phosphoglycerate mutase (1992), Eur. J. Biochem., 207, 709-714.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.11 | 2,3-diphosphoglycerate | Km: 0.0081, wild-type enzyme, Km: 0.087, H181A-mutant | Saccharomyces cerevisiae |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.2.11 | - |
Saccharomyces cerevisiae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.2.11 | H181A | His181 is substituted by Ala in site-directed mutagenesis. The resulting enzyme has a reduced activity and still requires 2,3-diphosphoglycerate. His181 seems to be important for cofactor-binding | Saccharomyces cerevisiae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.11 | 0.026 | - |
3-phosphoglycerate | H181A mutant | Saccharomyces cerevisiae | |
| 5.4.2.11 | 0.74 | - |
3-phosphoglycerate | wild-type enzyme | Saccharomyces cerevisiae | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.2.11 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.2.11 | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.11 | 0.078 | - |
3-phosphoglycerate | mutant H181A | Saccharomyces cerevisiae | |
| 5.4.2.11 | 490 | - |
3-phosphoglycerate | wild-type enzyme | Saccharomyces cerevisiae | |
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