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Literature summary extracted from

  • Yorifuji, T.; Kobayashi, T.; Tabuchi, A.; Shiritani, Y.; Yonaha, K.
    Distribution of amidinohydrolases among Pseudomonas and comparative studies of some purified enzymes by one-dimensional peptide mapping (1983), Agric. Biol. Chem., 47, 2825-2830.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.3.7 L-arginine induced by Pseudomonas sp.
3.5.3.7 L-arginine strain PAO1 not induced Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.2 38000
-
 4 * 38000, SDS-PAGE Pseudomonas sp.
3.5.3.7 35000
-
 6 * 35000, SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.2 guanidinoacetate + H2O Pseudomonas sp.
-
 glycine + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O Pseudomonas putida
-
 glycine + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O Pseudomonas sp. ATCC 14676
-
 glycine + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas fluorescens
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas aeruginosa
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas sp.
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas putida
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas aeruginosa PAO1(ATTC 15692)
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas sp. ATCC 14676
-
 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O Pseudomonas aeruginosa PAO 1
-
 4-Aminobutyrate + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.2 Pseudomonas putida
-
 ATCC 12633
-
3.5.3.2 Pseudomonas sp.
-
-
-
3.5.3.2 Pseudomonas sp. ATCC 14676
-
-
-
3.5.3.7 Pseudomonas aeruginosa
-
 PAO1(ATTC 15692)
-
3.5.3.7 Pseudomonas aeruginosa PAO1(ATTC 15692)
-
 PAO1(ATTC 15692)
-
3.5.3.7 Pseudomonas fluorescens
-
 ATCC 13525
-
3.5.3.7 Pseudomonas putida
-
 P2 (ATCC 25571)
-
3.5.3.7 Pseudomonas sp.
-
-
-
3.5.3.7 Pseudomonas sp. ATCC 14676
-
-
-
3.5.3.17 Pseudomonas aeruginosa
-
-
-
3.5.3.17 Pseudomonas aeruginosa
-
 ATCC 10145
-
3.5.3.17 Pseudomonas aeruginosa PAO 1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.2
-
 Pseudomonas sp.
3.5.3.7
-
 Pseudomonas fluorescens
3.5.3.7
-
 Pseudomonas aeruginosa
3.5.3.7
-
 Pseudomonas sp.
3.5.3.7
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.2 0.39
-
 ATCC 12633, GA induced Pseudomonas putida
3.5.3.7 287
-
-
 Pseudomonas sp.
3.5.3.7 910
-
 PAO1, purified enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.2 guanidinoacetate + H2O
-
 Pseudomonas sp. glycine + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O
-
 Pseudomonas putida glycine + urea
-
 ?
3.5.3.2 guanidinoacetate + H2O
-
 Pseudomonas sp. ATCC 14676 glycine + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas fluorescens 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas aeruginosa 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas sp. 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas putida 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas aeruginosa PAO1(ATTC 15692) 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas sp. ATCC 14676 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O
-
 Pseudomonas aeruginosa PAO 1 4-Aminobutyrate + urea
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.2 tetramer 4 * 38000, SDS-PAGE Pseudomonas sp.
3.5.3.7 hexamer 6 * 35000, SDS-PAGE Pseudomonas sp.