Literature summary extracted from

  • Sakai, K.; Oshima, K.; Moriguchi, M.
    Purification and characterization of N-acyl-D-glutamate amidohydrolase from Pseudomonas sp. strain 5f-1 (1991), Appl. Environ. Microbiol., 57, 2540-2543.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.82 acetate
-
Pseudomonas sp.

General Stability

EC Number General Stability Organism
3.5.1.82 Na+, K+, Mg2+, Ba2+ or glycerol stabilizes Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.82 Cu2+
-
Pseudomonas sp.
3.5.1.82 EDTA
-
Pseudomonas sp.
3.5.1.82 Fe3+
-
Pseudomonas sp.
3.5.1.82 Hg2+
-
Pseudomonas sp.
3.5.1.82 Zn2+
-
Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.82 6.67
-
N-Acetyl-D-Glu
-
Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.82 55000
-
1 * 55000, SDS-PAGE Pseudomonas sp.
3.5.1.82 44000
-
gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.82 additional information Pseudomonas sp. the enzyme is inducibly produced by D-isomers of N-acetylglutamate, Glu, Asp, and Asn ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.82 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.82
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.82 589
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.82 additional information the enzyme is inducibly produced by D-isomers of N-acetylglutamate, Glu, Asp, and Asn Pseudomonas sp. ?
-
?
3.5.1.82 N-Formyl-D-Glu + H2O
-
Pseudomonas sp. Formate + D-Glu
-
?
3.5.1.82 N-Acetyl-D-Glu + H2O 58% of the activity with N-formyl-D-Glu Pseudomonas sp. acetate + D-Glu
-
?
3.5.1.82 N-Propionyl-D-Glu + H2O 16% of the activity with N-formyl-D-Glu Pseudomonas sp. Propanoate + D-Glu
-
?
3.5.1.82 N-Butyl-D-Glu + H2O 21% of the activity with N-formyl-D-Glu Pseudomonas sp. Butanoate + D-Glu
-
?
3.5.1.82 N-Chloroacetyl-D-Glu + H2O 7.5% of the activity with N-formyl-D-Glu Pseudomonas sp. Chloroacetate + D-Glu
-
?
3.5.1.82 Glycyl-D-Glu + H2O 0.79% of the activity with N-formyl-D-Glu Pseudomonas sp. Gly + D-Glu
-
?

Subunits

EC Number Subunits Comment Organism
3.5.1.82 monomer 1 * 55000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.82 45
-
-
Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.82 48
-
10 min, in presence of Mg2+, 50% loss of activity Pseudomonas sp.
3.5.1.82 41
-
10 min, in absence of a stabilizer, 50% loss of activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.82 6.5 7.5
-
Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.82 9 9.5 45°C, quite stable, regardless of the presence or absence of MgCl2 Pseudomonas sp.