EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.82 | acetate | - |
Pseudomonas sp. |
EC Number | General Stability | Organism |
---|---|---|
3.5.1.82 | Na+, K+, Mg2+, Ba2+ or glycerol stabilizes | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.82 | Cu2+ | - |
Pseudomonas sp. | |
3.5.1.82 | EDTA | - |
Pseudomonas sp. | |
3.5.1.82 | Fe3+ | - |
Pseudomonas sp. | |
3.5.1.82 | Hg2+ | - |
Pseudomonas sp. | |
3.5.1.82 | Zn2+ | - |
Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.82 | 6.67 | - |
N-Acetyl-D-Glu | - |
Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.82 | 44000 | - |
gel filtration | Pseudomonas sp. |
3.5.1.82 | 55000 | - |
1 * 55000, SDS-PAGE | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.82 | additional information | Pseudomonas sp. | the enzyme is inducibly produced by D-isomers of N-acetylglutamate, Glu, Asp, and Asn | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.82 | Pseudomonas sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.82 | - |
Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.82 | 589 | - |
- |
Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.82 | Glycyl-D-Glu + H2O | 0.79% of the activity with N-formyl-D-Glu | Pseudomonas sp. | Gly + D-Glu | - |
? | |
3.5.1.82 | additional information | the enzyme is inducibly produced by D-isomers of N-acetylglutamate, Glu, Asp, and Asn | Pseudomonas sp. | ? | - |
? | |
3.5.1.82 | N-Acetyl-D-Glu + H2O | 58% of the activity with N-formyl-D-Glu | Pseudomonas sp. | acetate + D-Glu | - |
? | |
3.5.1.82 | N-Butyl-D-Glu + H2O | 21% of the activity with N-formyl-D-Glu | Pseudomonas sp. | Butanoate + D-Glu | - |
? | |
3.5.1.82 | N-Chloroacetyl-D-Glu + H2O | 7.5% of the activity with N-formyl-D-Glu | Pseudomonas sp. | Chloroacetate + D-Glu | - |
? | |
3.5.1.82 | N-Formyl-D-Glu + H2O | - |
Pseudomonas sp. | Formate + D-Glu | - |
? | |
3.5.1.82 | N-Propionyl-D-Glu + H2O | 16% of the activity with N-formyl-D-Glu | Pseudomonas sp. | Propanoate + D-Glu | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.82 | monomer | 1 * 55000, SDS-PAGE | Pseudomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.82 | 45 | - |
- |
Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.82 | 41 | - |
10 min, in absence of a stabilizer, 50% loss of activity | Pseudomonas sp. |
3.5.1.82 | 48 | - |
10 min, in presence of Mg2+, 50% loss of activity | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.82 | 6.5 | 7.5 | - |
Pseudomonas sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.82 | 9 | 9.5 | 45°C, quite stable, regardless of the presence or absence of MgCl2 | Pseudomonas sp. |