Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wakayama, M.; Katsuno, Y.; Hayashi, S.; Miyamoto, Y.; Sakai, K.; Moriguchi, M.
    Cloning and sequencing of a gene encoding D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 and expression of the gene in Escherichia coli (1995), Biosci. Biotechnol. Biochem., 59, 2115-2119.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.81 expression in Escherichia coli Achromobacter xylosoxidans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.81 Co2+ 1 mM Achromobacter xylosoxidans
3.5.1.81 Cu2+ 1 mM, 98% inhibition Achromobacter xylosoxidans
3.5.1.81 Fe2+ 1 mM Achromobacter xylosoxidans
3.5.1.81 Ni2+ 1 mM Achromobacter xylosoxidans
3.5.1.81 Zn2+ 1 mM, 94% inhibition Achromobacter xylosoxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.81 45000
-
 gel filtration Achromobacter xylosoxidans
3.5.1.81 51918
-
 x * 51918, calculation from nucleotide sequence Achromobacter xylosoxidans
3.5.1.81 52000
-
 x * 52000, SDS-PAGE Achromobacter xylosoxidans

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.81 Achromobacter xylosoxidans
-
 subsp. xylosoxydans A-6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.81 recombinant enzyme Achromobacter xylosoxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.81 1000
-
-
 Achromobacter xylosoxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.81 N-acetyl-D-Leu + H2O
-
 Achromobacter xylosoxidans acetate + D-Leu
-
 ?
3.5.1.81 N-Acetyl-D-Met + H2O
-
 Achromobacter xylosoxidans acetate + D-Met
-
 ?
3.5.1.81 N-Acetyl-D-norleucine + H2O
-
 Achromobacter xylosoxidans acetate + D-norleucine
-
 ?
3.5.1.81 N-Acetyl-D-Phe + H2O
-
 Achromobacter xylosoxidans acetate + D-Phe
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.81 ? x * 52000, SDS-PAGE Achromobacter xylosoxidans
3.5.1.81 ? x * 51918, calculation from nucleotide sequence Achromobacter xylosoxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.81 50
-
-
 Achromobacter xylosoxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.81 7 8
-
 Achromobacter xylosoxidans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.81 7 8 optimal stability between pH 7 and pH 8 Achromobacter xylosoxidans