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Literature summary extracted from

  • Black, T.D.; Briggs, B.S.; Evans, R.; Muth, W.L.; Vangala, S.; Zmijewski, M.J.
    o-Phthalyl amidase in the synthesis of loracarbef: process development using this novel biocatalyst (1996), Biotechnol. Lett., 18, 875-880.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.1.79 synthesis alternative chemical route in removing the phthalimido protecting group Xanthobacter agilis

General Stability

EC Number General Stability Organism
3.5.1.79 pH-stability and temperature-stability is improved significantly by increasing ionic strength of the buffer Xanthobacter agilis
3.5.1.79 the enzyme stability is optimal at salt concentrations of 0.2 M and above Xanthobacter agilis
3.5.1.79 the substrate phthalimido carbacephem stablilizes the enzyme Xanthobacter agilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.79 49000
-
 1 * 49000, SDS-PAGE Xanthobacter agilis
3.5.1.79 49724
-
 1 * 49724, calculation from nucleotide sequence Xanthobacter agilis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.79 Xanthobacter agilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.79 large scale Xanthobacter agilis

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.79 A phthalylamide + H2O = phthalic acid + a substituted amine mechanism Xanthobacter agilis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.79 9
-
-
 Xanthobacter agilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.79 2-Carboxybenzoylamine-D-Phe-L-Ala
-
 Xanthobacter agilis ?
-
 ?
3.5.1.79 2-Carboxybenzoylamine-D-phenylglycine-methylester
-
 Xanthobacter agilis ?
-
 ?
3.5.1.79 2-Carboxybenzoylamine-L-Phe-L-Ala
-
 Xanthobacter agilis ?
-
 ?
3.5.1.79 additional information broad specificity for o-phthalylated amides, absolute requirement for the o-phthalyl protecting group Xanthobacter agilis ?
-
 ?
3.5.1.79 o-Phthalyl carbacephem
-
 Xanthobacter agilis Loracarbef + phthalic acid
-
 ?
3.5.1.79 [4-[(2-Carboxybenzoyl)amino]-1H-imidazol-1-yl]octanoic acid
-
 Xanthobacter agilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.79 monomer 1 * 49000, SDS-PAGE Xanthobacter agilis
3.5.1.79 monomer 1 * 49724, calculation from nucleotide sequence Xanthobacter agilis