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Literature summary extracted from

  • Ogawa, J.; Chung, M.C.M.; Hida, S.; Yamada, H.; Shimizu, S.
    Thermostable N-carbamoyl-D-amino acid amidohydrolase: screening, purification and characterization (1994), J. Biotechnol., 38, 11-19.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.77 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Blastobacter sp.
3.5.1.77 Ag+
-
 Blastobacter sp.
3.5.1.77 Cd2+
-
 Blastobacter sp.
3.5.1.77 Co2+
-
 Blastobacter sp.
3.5.1.77 Cu2+
-
 Blastobacter sp.
3.5.1.77 D-Phe
-
 Blastobacter sp.
3.5.1.77 Hg2+
-
 Blastobacter sp.
3.5.1.77 N-carbamoyl-D-Phe
-
 Blastobacter sp.
3.5.1.77 NaAsO2
-
 Blastobacter sp.
3.5.1.77 NEM
-
 Blastobacter sp.
3.5.1.77 NH4+
-
 Blastobacter sp.
3.5.1.77 PCMB
-
 Blastobacter sp.
3.5.1.77 Zn2+
-
 Blastobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.77 0.36
-
 N-carbamoyl-D-Leu
-
 Blastobacter sp.
3.5.1.77 0.41
-
 N-carbamoyl-D-Val
-
 Blastobacter sp.
3.5.1.77 0.5
-
 N-carbamoyl-D-Phe
-
 Blastobacter sp.
3.5.1.77 0.71
-
 N-Carbamoyl-DL-Met
-
 Blastobacter sp.
3.5.1.77 0.79
-
 N-carbamoyl-D-norleucine
-
 Blastobacter sp.
3.5.1.77 0.88
-
 N-Carbamoyl-D-phenylglycine
-
 Blastobacter sp.
3.5.1.77 1.7
-
 N-carbamoyl-D-p-hydroxyphenylglycine
-
 Blastobacter sp.
3.5.1.77 2.4
-
 N-carbamoyl-D-Ser
-
 Blastobacter sp.
3.5.1.77 4
-
 N-carbamoyl-D-Ala
-
 Blastobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.77 40000
-
 3 * 40000, SDS-PAGE Blastobacter sp.
3.5.1.77 111000 120000 gel filtration Blastobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.77 Blastobacter sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.77
-
 Blastobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.77 additional information
-
-
 Blastobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.77 DL-5-(p-Hydroxyphenyl)hydantoin + H2O
-
 Blastobacter sp. ?
-
 ?
3.5.1.77 additional information the enzyme does not hydrolyze beta-ureidopropionate Blastobacter sp. ?
-
 ?
3.5.1.77 N-carbamoyl-D-Ala + H2O 29% of the activity with N-carbamoyl-D-Phe Blastobacter sp. D-Ala + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Leu + H2O 23% of the activity with N-carbamoyl-D-Phe Blastobacter sp. D-Leu + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-norleucine + H2O
-
 Blastobacter sp. D-norleucine + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-p-hydroxyphenylglycine + H2O 132% of the activity with N-carbamoyl-D-Phe Blastobacter sp. D-p-hydroxyphenylglycine + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Phe + H2O
-
 Blastobacter sp. D-Phe + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-phenylglycine + H2O 170% of the activity with N-carbamoyl-D-Phe Blastobacter sp. D-phenylglycine + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Ser + H2O 10% of the activity with N-carbamoyl-D-Phe Blastobacter sp. D-Ser + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Trp + H2O 18% of the activity with N-carbamoyl-D-Phe Blastobacter sp. D-Trp + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-Val + H2O 55% of the activity with N-carbamoyl-D-Phe Blastobacter sp. D-Val + NH3 + CO2
-
 ?
3.5.1.77 N-Carbamoyl-DL-2-amino-n-butyric acid + H2O 48% of the activity with N-carbamoyl-D-Phe Blastobacter sp. ?
-
 ?
3.5.1.77 N-Carbamoyl-DL-Met + H2O 84% of the activity with N-carbamoyl-D-Phe Blastobacter sp. ?
-
 ?
3.5.1.77 N-Carbamoyl-DL-norleucine + H2O
-
 Blastobacter sp. ?
-
 ?
3.5.1.77 N-Carbamoyl-DL-norvaline + H2O 26% of the activity with N-carbamoyl-D-Phe Blastobacter sp. ?
-
 ?
3.5.1.77 N-Carbamoyl-DL-Thr + H2O 18% of the activity with N-carbamoyl-D-Phe Blastobacter sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.77 trimer 3 * 40000, SDS-PAGE Blastobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.77 55
-
-
 Blastobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.77 30 60 30°C: about 35% of maximal activity, 20°C: about 20% of maximal activity Blastobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.77 40
-
 pH 6.0-9.0, 40 min, less than 10% loss of activity Blastobacter sp.
3.5.1.77 50
-
 1 h, stable up to Blastobacter sp.
3.5.1.77 55
-
 1 h, 25% loss of activity Blastobacter sp.