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Literature summary extracted from

  • Sugai, M.; Fujiwara, T.; Akiyama, T.; Ohara, M.; Komatsuzawa, H.; Inoue, S.; Suginaka, H.
    Purification and molecular characterization of glycylglycine endopeptidase produced by Staphylococcus capitis EPK1 (1997), J. Bacteriol., 179, 1193-1202.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.75
-
 Staphylococcus capitis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.75 1,10-phenanthroline
-
 Staphylococcus capitis
3.4.24.75 Cu2+
-
 Staphylococcus capitis
3.4.24.75 diethyldicarbonate
-
 Staphylococcus capitis
3.4.24.75 iodoacetate
-
 Staphylococcus capitis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.75 extracellular
-
 Staphylococcus capitis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.75 Na+ stimulation Staphylococcus capitis
3.4.24.75 NH4+ stimulation Staphylococcus capitis
3.4.24.75 Zn2+ metalloprotease, 1 mol of Zn2+ per mol of enzyme, atomic absorption spectroscopy Staphylococcus capitis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.75 35600
-
 mature enzyme, calculation from nucleotide sequence Staphylococcus capitis
3.4.24.75 39310
-
 proenzyme, calculation from nucleotide sequence Staphylococcus capitis
3.4.24.75 48000
-
 SDS-PAGE in presence and absence of reducing agents Staphylococcus capitis

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.75 Staphylococcus capitis
-
 EPK1
-
3.4.24.75 Staphylococcus capitis EPK1
-
 EPK1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.75
-
 Staphylococcus capitis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.75 additional information
-
-
 Staphylococcus capitis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.75 additional information no hydrolysis of beta-casein Staphylococcus capitis ?
-
 ?
3.4.24.75 additional information no hydrolysis of beta-casein Staphylococcus capitis EPK1 ?
-
 ?
3.4.24.75 Peptidoglycan + H2O hydrolyis of pentaglycine interpeptide bridges of Staphylococcal peptidoglycan Staphylococcus capitis Fragments of peptidoglycan
-
 ?
3.4.24.75 Peptidoglycan + H2O hydrolyis of pentaglycine interpeptide bridges of Staphylococcal peptidoglycan Staphylococcus capitis EPK1 Fragments of peptidoglycan
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.75 7 9
-
 Staphylococcus capitis