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Literature summary extracted from

  • Thumm, G.; Götz, F.
    Studies on prolysostaphin processing and characterization of lysostaphin immunity factor (Lif) of Staphylococcus simulans biovar staphylolyticus (1997), Mol. Microbiol., 23, 1251-1265.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.75 expression in Escherichia coli with pUC19 as vector Staphylococcus simulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.75 additional information not inhibitory is trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane, i.e. E-64 Staphylococcus simulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.75 extracellular
-
 Staphylococcus simulans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.75 49120
-
 prolysostaphin, calculation from nucleotide sequence. Prolysostaphin is processed to the mature enzyme by an extracellular cysteine protease Staphylococcus simulans
3.4.24.75 64000 68000 the overestimated value is due to a high glutamate content. Prolysostaphin is processed to the mature enzyme by an extracellular cysteine protease Staphylococcus simulans
3.4.24.75 64000 68000 SDS-PAGE of prolysostaphin Staphylococcus simulans

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.75 Staphylococcus simulans
-
 biovar staphylolyticus, ATCC 1362
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.75
-
 Staphylococcus simulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.75 additional information
-
 169 U/mg (prolysostaphin), 760 U/mg (mature lysostaphin), 1 unit is defined as the amount of enzyme that causes 50% reduction of turbidity in 10 min at 37 C of an inactivated Staphylococcus carnosus TM300 cell suspension Staphylococcus simulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.75 Peptidoglycan + H2O hydrolyis of pentaglycine interpeptide bridges of Staphylococcal peptidoglycan Staphylococcus simulans Fragments of peptidoglycan
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.75 monomer 1 * 64000-68000, prolysostaphin, the overestimated value is due to a high glutamate content Staphylococcus simulans