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Literature summary extracted from

  • Guan, A.L.; Retzios, A.D.; Henderson, G.N.; Markland, F.S.
    Purification and characterization of a fibrinolytic enzyme from venom of the southern copperhead snake (Agkistrodon contortrix contortrix) (1991), Arch. Biochem. Biophys., 289, 197-207.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.72 1,10-phenanthroline
-
 Agkistrodon contortrix contortrix
3.4.24.72 EDTA
-
 Agkistrodon contortrix contortrix
3.4.24.72 additional information not: DFP, soybean trypsin inhibitor, trasylol, PCMB Agkistrodon contortrix contortrix
3.4.24.72 Tetraethylenepentamine
-
 Agkistrodon contortrix contortrix
3.4.24.72 Zincov
-
 Agkistrodon contortrix contortrix

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.72 Zinc zinc metalloproteinase Agkistrodon contortrix contortrix
3.4.24.72 Zinc enzyme contains 1 mol of zinc per mol of protein Agkistrodon contortrix contortrix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.72 22540
-
 Agkistrodon contortrix, calculation from amino acid sequence Agkistrodon contortrix contortrix

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.72 Agkistrodon contortrix contortrix
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.72 additional information contains little or no carbohydrate Agkistrodon contortrix contortrix

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.72
-
 Agkistrodon contortrix contortrix

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.72 venom
-
 Agkistrodon contortrix contortrix
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.72 additional information
-
-
 Agkistrodon contortrix contortrix

Storage Stability

EC Number Storage Stability Organism
3.4.24.72 4°C or -20°C, stable for 6 months, 20-30% loss of activity after 1 year Agkistrodon contortrix contortrix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.72 azocasein + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 Insulin B-chain + H2O cleavage between Ala14 and Leu15 Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 additional information no activity against a series of chromogenic 4-nitroanilide substrates Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 additional information exhibits little if any hemorrhagic activity Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 additional information does not activate plasminogen Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 additional information direct fibrinolytic activity Agkistrodon contortrix contortrix ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.72 ? x * 23000-24000, Agkistrodon contortrix, SDS-PAGE under reducing and nonreducing condition Agkistrodon contortrix contortrix

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.72 5.5
-
 6 h, 30-35% loss of activity Agkistrodon contortrix contortrix
3.4.24.72 6.5
-
 optimal pH for stability Agkistrodon contortrix contortrix
3.4.24.72 9
-
 6 h, 45-50% loss of activity Agkistrodon contortrix contortrix