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Literature summary extracted from

  • Xu, D.; Emoto, N.; Giaid, A.; Slaughter, C.; Kaw, S.; deWit, D.; Yanagisawa, M.
    ECE-1: a membrane-bound metalloprotease that catalyzes the proteolytic activation of big endothelin-1 (1994), Cell, 78, 473-485.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.71 bovine Bos taurus
3.4.24.71 expressed in Chinese hamster ovary CHO-K1 cells Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.71 1,10-phenanthroline
-
 Bos taurus
3.4.24.71 EDTA 0.03 mM, Zn2+ restores, Cu2+, Co2+, Mn2+ or Fe2+ partially restores, not Ca2+ or Mg2+ Bos taurus
3.4.24.71 FR901533 specific ECE-1 inhibitor, in vitro not in vivo Bos taurus
3.4.24.71 additional information leupeptin, chymostatin, pepstatin A, E-64, p-chloromercuriphenylsulfonic acid, N-ethylmaleimide or 4-amidinophenylmethyl sulfonylfluoride; no inhibition by thiorphan, captopril Bos taurus
3.4.24.71 phosphoramidon 0.1 mM, in vivo and in vitro Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.71 membrane integral membrane protein Bos taurus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.71 85610
-
 bovine, deduced from nucleotide sequence Bos taurus
3.4.24.71 126000
-
 1 * 126000, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.71 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.71 glycoprotein
-
 Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.71 to near homogeneity Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.71 adrenal cortex
-
 Bos taurus
-
3.4.24.71 endothelium vascular Bos taurus
-
3.4.24.71 additional information tissue distribution of mRNA Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.71 Human big endothelin 1 + H2O best substrate Bos taurus Endothelin 1 + big endothelin 1(21-38)
-
 ?
3.4.24.71 Human big endothelin 1 + H2O cleavage site: Trp21-Val22 Bos taurus Endothelin 1 + big endothelin 1(21-38)
-
 ?
3.4.24.71 Human big endothelin 2 + H2O hydrolysis at about 40% the rate of big endothelin1 Bos taurus ?
-
 ?
3.4.24.71 Human big endothelin 3 + H2O hydrolysis at about 30% the rate of big endothelin1 Bos taurus ?
-
 ?
3.4.24.71 Human big endothelin 3 + H2O big endothelin 3(1-41)amide Bos taurus ?
-
 ?
3.4.24.71 additional information no substrate is the big endothelin 1(1-31) fragment Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.71 monomer 1 * 126000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.71 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.71 6.8
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.71 5.8 7.8 about half-maximal activity at pH 5.8 and 7.8 Bos taurus