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Literature summary extracted from

  • Schiavo, G.; Malizio, C.; Trimble, W.S.; Polverino de Laureto, P.; Milan, G.; Sugiyama, H.; Johnson, E.A.; Montecucco, C.
    Botulinum G neurotoxin cleaves VAMP/synaptobrevin at a single Ala-Ala peptide bond (1994), J. Biol. Chem., 269, 20213-20216.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.69 1,10-phenanthroline
-
 Clostridium botulinum
3.4.24.69 captopril
-
 Clostridium botulinum
3.4.24.69 EDTA
-
 Clostridium botulinum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.69 Zinc activation requires reduction of interchain disulfide bond Clostridium botulinum
3.4.24.69 Zinc contains zinc binding motif of metalloendopeptidases His-Glu-X-X-His Clostridium botulinum
3.4.24.69 Zinc zinc-dependent endopeptidase Clostridium botulinum

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.69 Clostridium botulinum
-
 serotypes BoNT/A, BoNT/B
-
3.4.24.69 Clostridium botulinum
-
 BoNT/C, BoNT/D
-
3.4.24.69 Clostridium botulinum
-
 type G strain
-
3.4.24.69 Clostridium botulinum type G
-
 type G strain
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.69 additional information catalytic activity requires reduction of the single interchain disulfide bond of the neurotoxin Clostridium botulinum ?
-
 ?
3.4.24.69 additional information the clostridial neurotoxins differ from other proteases in the recognition of the tertiary structure of the target rather than the sequence of the peptide bond to be cleaved Clostridium botulinum ?
-
 ?
3.4.24.69 additional information synaptotagmin, synaptophysin Clostridium botulinum ?
-
 ?
3.4.24.69 additional information catalytic activity requires reduction of the single interchain disulfide bond of the neurotoxin Clostridium botulinum type G ?
-
 ?
3.4.24.69 additional information the clostridial neurotoxins differ from other proteases in the recognition of the tertiary structure of the target rather than the sequence of the peptide bond to be cleaved Clostridium botulinum type G ?
-
 ?
3.4.24.69 additional information synaptotagmin, synaptophysin Clostridium botulinum type G ?
-
 ?
3.4.24.69 Recombinant glutathione S-methyltransferase VAMP-2 fusion protein + H2O
-
 Clostridium botulinum Hydrolyzed recombinant glutathione S-methyltransferase VAMP-2 fusion protein 2 proteolytic fragments, MW 36000 and MW 6000 ?
3.4.24.69 Synaptobrevin + H2O i.e. VAMP, neuronal vesicle-associated membrane protein, MW 19000, with 2 isoforms in human, chicken, in rat brain: synaptobrevin/VAMP-1 and synaptobrevin/VAMP-2, cleaves at Gln76-Phe77, the same site as botulin neurotoxin B Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O serotype BoNT/B: cleavage at -Asp-Gln-+-Lys-Leu-, serotype BoNT/G: cleavage at Ala83-Ala84 (VAMP-1), Ala81-Ala82 (VAMP-2) Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O carrying synaptobrevin/VAMP-2 Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O both isoforms are cleaved at the same rate Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O carrying Val76 instead of Gln76 is not hydrolyzed by serotype BoNT/B Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O highly specific neurotoxins Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O i.e. VAMP, neuronal vesicle-associated membrane protein, MW 19000, with 2 isoforms in human, chicken, in rat brain: synaptobrevin/VAMP-1 and synaptobrevin/VAMP-2, cleaves at Gln76-Phe77, the same site as botulin neurotoxin B Clostridium botulinum type G Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O serotype BoNT/B: cleavage at -Asp-Gln-+-Lys-Leu-, serotype BoNT/G: cleavage at Ala83-Ala84 (VAMP-1), Ala81-Ala82 (VAMP-2) Clostridium botulinum type G Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptosome-associated protein + H2O no substrate of serotype BoNT/G Clostridium botulinum Hydrolyzed synaptosome-associated protein
-
 ?
3.4.24.69 Syntaxin + H2O no substrate of serotype BoNT/G Clostridium botulinum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.69 37
-
 assay at Clostridium botulinum