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Literature summary extracted from
- Purification and partial characterization of high choriolytic enzyme (HCE), a component of the hatching enzyme of the teleost, Oryzias latipes (1989), J. Biochem., 105, 204-211.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.67 | EDTA | succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide as substrate, 0.8 mM ZnCl2 restores or 80 mM CuCl2 partially restores, not NiCl2, MgCl2 or CaCl2 | Oryzias latipes |  |
| 3.4.24.67 | additional information | no inhibition by monoiodoacetamide, monoiodoacetic acid or diisopropyl fluorophosphate | Oryzias latipes |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.24.67 | soluble | - |
Oryzias latipes | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.67 | Calcium | 0.00286-0.00296 mg/mg protein, inductively coupled plasma emission analysis | Oryzias latipes | |
| 3.4.24.67 | Magnesium | 0.00063-0.00076 mg/mg protein, inductively coupled plasma emission analysis | Oryzias latipes | |
| 3.4.24.67 | additional information | copper barely detectable by atom absorption analysis and no cobalt, iron or manganese detected by inductively coupled plasma emission analysis | Oryzias latipes | |
| 3.4.24.67 | Zinc | 0.00127-0.00143 mg/mg protein, inductively coupled plasma emission analysis | Oryzias latipes | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.67 | additional information | - |
amino acid composition of isomers HCE-1 and HCE-2 | Oryzias latipes |
| 3.4.24.67 | 20000 | - |
Oryzias latipes, deduced from sedimentation coefficient | Oryzias latipes |
| 3.4.24.67 | 23010 | - |
Oryzias latipes, HCE-1, estimation on the basis of amino acid composition | Oryzias latipes |
| 3.4.24.67 | 23470 | - |
Oryzias latipes, HCE-2, estimation on the basis of amino acid composition | Oryzias latipes |
| 3.4.24.67 | 24000 | - |
1 * 24000, Oryzias latipes, SDS-PAGE | Oryzias latipes |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.67 | chorion + H2O | Oryzias latipes | concerted action with other component of hatching enzyme, i.e. choriolysin L, EC 3.4.24.66 | ? | - |
? | |
| 3.4.24.67 | chorion + H2O | Oryzias latipes | embryo-secreted enzyme | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.67 | Oryzias latipes | - |
medaka, teleost fish, orange-red variety | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.67 | 2 isomers: HCE-1 and HCE-2, separable by HPLC ion exchange chromatography | Oryzias latipes |
| 3.4.24.67 | from hatching liquid | Oryzias latipes |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.67 | blastula | - |
Oryzias latipes | - |
| 3.4.24.67 | embryo | embryo-secreted enzyme | Oryzias latipes | - |
| 3.4.24.67 | hatching liquid | i.e. culture medium of blastulae | Oryzias latipes | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.67 | additional information | - |
- |
Oryzias latipes |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.24.67 | 0°C, in the presence of Ca2+ and Mg2+ in addition to Zn2+, 9 weeks | Oryzias latipes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.67 | Benzoyl-Arg 4-methylcoumarin 7-amide + H2O | hydrolysis at about 3% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide | Oryzias latipes | ? | - |
? | |
| 3.4.24.67 | Benzyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide + H2O | hydrolysis at about 2% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide | Oryzias latipes | ? | - |
? | |
| 3.4.24.67 | Chorion + H2O | - |
Oryzias latipes | Swollen chorion + ? | - |
? | |
| 3.4.24.67 | chorion + H2O | concerted action with other component of hatching enzyme, i.e. choriolysin L, EC 3.4.24.66 | Oryzias latipes | ? | - |
? | |
| 3.4.24.67 | chorion + H2O | embryo-secreted enzyme | Oryzias latipes | ? | - |
? | |
| 3.4.24.67 | additional information | exhibits choriolytic and proteolytic activity | Oryzias latipes | ? | - |
? | |
| 3.4.24.67 | additional information | N-tert-butoxycarbonyl-Ile-Glu-Gly-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Ser-Thr-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Glu-Lys-Lys 4-methylcoumarin 7-amide, succinyl-Gly-Pro 4-methylcoumarin 7-amide or Leu 4-methylcoumarin 7-amide | Oryzias latipes | ? | - |
? | |
| 3.4.24.67 | N-tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O | hydrolysis at about 12% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide | Oryzias latipes | ? | - |
? | |
| 3.4.24.67 | Peptidyl 4-methylcoumarin 7-amides + H2O | i.e. MCA-peptides | Oryzias latipes | ? | - |
? | |
| 3.4.24.67 | Succinyl-Ala-Ala-Pro-Phe 4-methylcoumarin 7-amide + H2O | hydrolysis at about 13% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide | Oryzias latipes | ? | - |
? | |
| 3.4.24.67 | Succinyl-Ala-Pro-Ala 4-methylcoumarin 7-amide + H2O | hydrolysis at about 45% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide | Oryzias latipes | ? | - |
? | |
| 3.4.24.67 | Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O | best substrate of peptidyl 4-methylcoumarin 7-amides | Oryzias latipes | Succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.67 | monomer | 1 * 24000, Oryzias latipes, SDS-PAGE | Oryzias latipes |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.67 | 30 | - |
assay at | Oryzias latipes |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.67 | 10 | 40 | 10 min, gradual decrease of choriolytic activity (10-30% loss) | Oryzias latipes |
| 3.4.24.67 | 25 | - |
4 days, in 50 mM Tris-HCl, 10 mM NaCl, pH 7.5, about 80% loss of choriolytic activity, 0.05% Brij-35 stabilizes, caseinolytic activity is less susceptible to long-term incubation | Oryzias latipes |
| 3.4.24.67 | 44 | - |
10 min, about 50% loss of choriolytic activity | Oryzias latipes |
| 3.4.24.67 | 50 | - |
10 min, about 90% loss of choriolytic activity | Oryzias latipes |
| 3.4.24.67 | 60 | - |
inactivation | Oryzias latipes |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.67 | additional information | - |
pI: 10.2 | Oryzias latipes |
| 3.4.24.67 | 8 | - |
caseinolytic activity | Oryzias latipes |
| 3.4.24.67 | 8.7 | - |
choriolytic activity | Oryzias latipes |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.67 | 6.5 | 8.9 | about 50% of maximal caseinolytic activity at pH 6.5 and 8.9 | Oryzias latipes |
| 3.4.24.67 | 7.9 | 9 | about 50% of maximal choriolytic activity at pH 7.9 and 9 | Oryzias latipes |
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