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Literature summary extracted from

  • Yasumasu, S.; Iuchi, I.; Yamagami, K.
    Isolation and some properties of low choriolytic enzyme (LCE), a component of the hatching enzyme of the teleost, Oryzias latipes (1989), J. Biochem., 105, 212-218.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.66 EDTA 0.5 mM, choriolytic and caseinolytic activity, 1 mM Zn2+ restores, 5 mM Cu2+ partially restores, not Ca2+ or Mg2+ Oryzias latipes
3.4.24.66 additional information no inhibition of caseinolytic activity by 1-10 mM IAA, monoiodoacetic acid or diisopropyl fluorophosphate Oryzias latipes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.66 soluble
-
Oryzias latipes
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.66 Calcium 0.00538 mg/mg protein, inductively coupled plasma emission analysis Oryzias latipes
3.4.24.66 Magnesium 0.00164 mg/mg protein, inductively coupled plasma emission analysis Oryzias latipes
3.4.24.66 additional information no cobalt, manganese or iron detected by inductively coupled plasma emission analysis Oryzias latipes
3.4.24.66 Zinc 0.00124 mg/mg protein, inductively coupled plasma emission analysis Oryzias latipes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.66 additional information
-
amino acid composition Oryzias latipes
3.4.24.66 25500
-
1 * 25500, Oryzias latipes, SDS-PAGE Oryzias latipes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.66 Swollen chorion + H2O Oryzias latipes i.e. inner layer of chorion that has been swollen by previous action of choriolysin H, EC 3.4.24.67, essential reaction in choriolysis, component of embryo-secreted hatching enzyme that digests egg envelope ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.66 Oryzias latipes
-
medaka, teleost fish, orange-red variety
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.66 from hatching liquid Oryzias latipes

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.66 hatching liquid i.e. culture medium of blastulae Oryzias latipes
-
3.4.24.66 hatching liquid taken immediately after larvae hatched on the 6th day Oryzias latipes
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.66 additional information
-
-
Oryzias latipes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.66 Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O hydrolysis at about 8% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide Oryzias latipes ?
-
?
3.4.24.66 casein + H2O
-
Oryzias latipes ?
-
?
3.4.24.66 additional information poor substrates are succinyl-Ala-Pro-Ala 4-methylcoumarin 7-amide, succinyl-Ala-Ala-Pro-Phe 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Leu-Ser-Thr-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Leu-Thr-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Val-Leu-Lys 4-methylcoumarin 7-amide, no substrates are intact chorion or Gly-Pro 4-methylcoumarin 7-amide Oryzias latipes ?
-
?
3.4.24.66 Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O best substrate of peptidyl 4-methylcoumarin 7-amides Oryzias latipes ?
-
?
3.4.24.66 Swollen chorion + H2O i.e. partially digested chorion Oryzias latipes ?
-
?
3.4.24.66 Swollen chorion + H2O i.e. inner layer of chorion that has been swollen by previous action of choriolysin H, EC 3.4.24.67, essential reaction in choriolysis, component of embryo-secreted hatching enzyme that digests egg envelope Oryzias latipes ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.24.66 ? 1 * 25500, Oryzias latipes, SDS-PAGE Oryzias latipes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.66 additional information
-
pI: 9.8 Oryzias latipes
3.4.24.66 8.5
-
caseinolytic activity Oryzias latipes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.66 7 9 about 50% of maximal caseinolytic activity at pH 6.5 and 8.9 Oryzias latipes