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Literature summary extracted from

  • Banda, M.J.; Werb, Z.
    Mouse macrophage elastase. Purification and characterization as a metalloproteinase (1981), Biochem. J., 193, 589-605.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.4.24.65 Buffers with high concentration of Ca2+ stabilize during purification Mus musculus
3.4.24.65 Dialysis against 25 mM Tris-HCl, pH 7.6 inactivates, Ca2+ restores activity Mus musculus
3.4.24.65 Purified enzyme, not partially purified preparation, unstable in either glycerol or sucrose velocity gradients Mus musculus
3.4.24.65 Stable to freeze-thawing Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.65 alpha2-Macroglobulin SDS abolishes inhibition Mus musculus
3.4.24.65 dithiothreitol
-
 Mus musculus
3.4.24.65 EDTA Zn2+ at a 1:10 ratio of Zn2+/EDTA protects and reverses, not Ca2+, no reversal by Co2+ or Mg2+ Mus musculus
3.4.24.65 EGTA less effective than EDTA, Ca2+ at a 3:1 ratio of Ca2+/EDTA protects and reverses partially, Zn2+ at a 1:10 ratio of Zn2+/EGTA protects and reverses Mus musculus
3.4.24.65 additional information diisopropylphosphorofluoridate, PCMB, NEM, mersalyl, soybean trypsin inhibitor, acetyltetraalanylchloromethane, phosphoramidon, glycoprotein tissue inhibitor, alpha1-proteinase inhibitor; no inhibition by PMSF (form B) Mus musculus
3.4.24.65 organic solvents enzyme form B, above 0.5% v/v Mus musculus
3.4.24.65 Rabbit antiserum against purified elastase form B
-
 Mus musculus
3.4.24.65 Zn2+ at a 3fold molar excess of Zn2+ over chelating agent Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.65 extracellular
-
 Mus musculus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.65 Ca2+ requirement Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.65 22000
-
 mouse, form B and C, gel filtration, sucrose density gradient centrifugation Mus musculus
3.4.24.65 57000
-
 mouse, form A, sucrose density gradient centrifugation, gel filtration Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.65 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.65 3 chromatographically distinct forms A, B and C, predominant form is B Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.65 macrophage tissue culture medium of colchicin-treated inflammatory peritoneal macrophages Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.65 additional information
-
-
 Mus musculus

Storage Stability

EC Number Storage Stability Organism
3.4.24.65 -80°C, in 50 mM Tris-HCl, pH 7.6, 150 mM NaCl, 50 mM CaCl2, at least 3 months Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.65 Elastin + H2O preferentially cleaves on amino-side of Leus, not Val Mus musculus More products of elastin hydrolysis are predominantly high MW peptides, MW 90000-100000 ?
3.4.24.65 additional information acetyl-Ala-Ala-Ala 4-nitroanilide or benzyloxycarbonyl-Ala 2-naphthyl esters Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.65 monomer 1 * 22000, mouse, form B and C, SDS-PAGE Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.65 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.65 8
-
 enzyme form B Mus musculus