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Literature summary extracted from

  • Yang, M.; Geli, V.; Oppliger, W.; Suda, K.; James, P.; Schatz, G.
    The MAS-encoded processing protease of yeast mitochondria. Interaction of the purified enzyme with signal peptides and a purified precursor protein (1991), J. Biol. Chem., 266, 6416-6423.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.64 1,10-phenanthroline
-
 Saccharomyces cerevisiae
3.4.24.64 additional information prepeptides pSynB2, p15 Saccharomyces cerevisiae
3.4.24.64 Prepeptide p25 derived from amino terminal of cytochrome oxidase subunit IV precursor, kinetics Saccharomyces cerevisiae
3.4.24.64 Prepeptide p34 derived from amino terminal of cytochrome oxidase subunit IV precursor, kinetics Saccharomyces cerevisiae
3.4.24.64 Prepeptide pSynC or pSynA2 less effective than p25 or p34 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.64 mitochondrion matrix Saccharomyces cerevisiae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.64 Co2+ metalloprotease Saccharomyces cerevisiae
3.4.24.64 Co2+ requirement (in vitro, in vivo not identified yet) Saccharomyces cerevisiae
3.4.24.64 Mn2+ requirement Saccharomyces cerevisiae
3.4.24.64 Mn2+ metalloprotease Saccharomyces cerevisiae
3.4.24.64 Mn2+ requirement (in vitro, in vivo not identified yet) Saccharomyces cerevisiae
3.4.24.64 additional information atomic absorption spectroscopy: purified MAS1/MAS2-holoenzyme lacks significant amounts of zinc, manganese or cobalt, probably lost during purification Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.64 additional information
-
 amino acid sequence comparison on the basis of statistical analysis, FASTP program Saccharomyces cerevisiae
3.4.24.64 48800
-
 1 * 48800 (MAS1) + 1 * 51800 (MAS2), Saccharomyces cerevisiae, calculated from amino acid sequence Saccharomyces cerevisiae
3.4.24.64 51800
-
 1 * 48800 (MAS1) + 1 * 51800 (MAS2), Saccharomyces cerevisiae, calculated from amino acid sequence Saccharomyces cerevisiae
3.4.24.64 100000
-
 sedimentation velocity analysis Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.64 additional information Saccharomyces cerevisiae removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.64 Saccharomyces cerevisiae
-
 overproducing strain VGA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.64 2 components: MAS1 and MAS2 Saccharomyces cerevisiae
3.4.24.64 if MAS1 is overproduced in the absence of MAS2 it is insoluble and not suitable for purification, it is therefore purified from the purified holoenzyme Saccharomyces cerevisiae
3.4.24.64 improved procedure Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.24.64 Release of N-terminal targetting peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2 mechanism Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.64 0.000001
-
 artificial fusion protein Saccharomyces cerevisiae
3.4.24.64 0.000003
-
 P25-peptide Saccharomyces cerevisiae
3.4.24.64 1.785
-
 strain JKR 102(YEp13-MAS1), overexpressing MAS1 Saccharomyces cerevisiae
3.4.24.64 2.35
-
 strain JKR 102 Saccharomyces cerevisiae
3.4.24.64 4.688
-
 strain JKR 102(pCF35-MAS2), overexpressing MAS2 Saccharomyces cerevisiae
3.4.24.64 23.44
-
 strain JKR 102(YEp13-MAS1 plus pCF35-MAS2), overexpressing MAS1 and MAS2 Saccharomyces cerevisiae
3.4.24.64 26.78
-
 strain VGA Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.64 additional information substrate binds to MAS2, not MAS1 enzyme component (photocrosslinking experiment) Saccharomyces cerevisiae ?
-
 ?
3.4.24.64 additional information removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes Saccharomyces cerevisiae ?
-
 ?
3.4.24.64 P-25 peptide + H2O matrix-targeting peptide containing cleavage site of authentic precursor protein Saccharomyces cerevisiae ?
-
 ?
3.4.24.64 Precytochrome b2-mouse dehydrofolate reductase fusion protein + H2O
-
 Saccharomyces cerevisiae 31-aminoacid presequence + cytochrome b2-mouse dehydrofolate reductase protein
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.64 dimer 1 * 48800 (MAS1) + 1 * 51800 (MAS2), Saccharomyces cerevisiae, calculated from amino acid sequence Saccharomyces cerevisiae