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Literature summary extracted from

  • Delporte, C.; Carvalho, K.M.; Leseney, A.M.; Winand, J.; Christophe, J.; Cohen, P.
    A new metallo- endopeptidase from human neuroblastoma NB-OK-1 cells which inactivates atrial natriuretic peptide by selective cleavage at the Ser123-Phe124 bond (1992), Biochem. Biophys. Res. Commun., 182, 158-164.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.60 1,10-phenanthroline metalloendopeptidase character Homo sapiens
3.4.24.60 benzamidine weak Homo sapiens
3.4.24.60 EDTA metalloendopeptidase character Homo sapiens
3.4.24.60 EGTA metalloendopeptidase character Homo sapiens
3.4.24.60 additional information no inhibition by N-tosyl-L-Phe chloromethyl ketone (i.e. TPCK); pepstatin, guanidylethylmercaptosuccinic acid (i.e. GEMSA), captopril, aprotinin, soybean trypsin inhibitor (i.e. STI), PMSF, IAA Homo sapiens
3.4.24.60 N-ethylmaleimide
-
Homo sapiens
3.4.24.60 p-chloromercuriphenylsulfonic acid
-
Homo sapiens
3.4.24.60 phosphoramidon not Homo sapiens
3.4.24.60 thiorphan not Homo sapiens
3.4.24.60 tosyl-Arg methyl ester i.e. TAME, weak Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.60 0.019
-
atrial natriuretic peptide
-
Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.60 extracellular
-
Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.60 additional information metalloendopeptidase Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.60 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.60 partial Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.60 neuroblastoma cell line NB-OK-1 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.60 angiotensin II + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe, cleavage site: Tyr4-Ile5 Homo sapiens ?
-
?
3.4.24.60 ANP-(103-126) + H2O i.e. atrial natriuretic peptide, cleavage sites: Xaa-Phe, Xaa-Leu or Xaa-Ile bonds Homo sapiens ANP-(103-123) + Phe124-Arg125-Tyr126
-
?
3.4.24.60 Asp-Ala-Asp-Thr-Cys-[D-Arg8]-kermit + H2O model substrate, derivative of kermit, cleavage site: Ser12-Phe13 Homo sapiens ?
-
?
3.4.24.60 atrial natriuretic peptide + H2O cleavage at Ser123-Phe124 Homo sapiens ?
-
?
3.4.24.60 bradykinin + H2O i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg, major cleavage site: Gly4-Phe5, minor site: Pro7-Pro8 Homo sapiens ?
-
?
3.4.24.60 Litorin + H2O minor sites: Gly6-His7 Homo sapiens ?
-
?
3.4.24.60 Litorin + H2O major cleavage sites: His7-Phe8 Homo sapiens ?
-
?
3.4.24.60 additional information no cleavage of analogous bonds in [Leu5]-enkephalin, oxytocin, vasopressin Homo sapiens ?
-
?
3.4.24.60 additional information cleavage sites: Xaa-Phe, Xaa-Leu, Xaa-Ile Homo sapiens ?
-
?
3.4.24.60 Neuromedin C + H2O Gly-Asn-His-Trp-Ala-Val-Gly-His-Leu-Met-NH2, major cleavage site: His8-Leu9, minor site: Gly7-His8 Homo sapiens ?
-
?
3.4.24.60 Somatostatin-14 + H2O i.e. Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys, cleavage site: Phe6-Phe7 within disulfide bridged segment yielding one single product Homo sapiens ?
-
?
3.4.24.60 Substance P + H2O major cleavage site: Gly9-Leu10, minor site: Phe7-Phe8 Homo sapiens ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.60 7
-
-
Homo sapiens