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Literature summary extracted from

  • Kurecki, T.; Laskowski, M.; Kress, L.F.
    Purification and some properties of two proteinases from Crotalus adamanteus venom that inactivate human alpha 1-proteinase inhibitor (1978), J. Biol. Chem., 253, 8340-8345.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.46 Crotalus adamanteus Crotalus adamanteus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.46 2-mercaptoethanol 2 mM Zn2+ protects at 2 mM 2-mercaptoethanol, 2 mM Zn2+ partially protects at 10-50 mM 2-mercaptoethanol, 2 mM Ca2+ partially protects at 2-10 mM 2-mercaptoethanol, no protection at 50 mM 2-mercaptoethanol Crotalus adamanteus
3.4.24.46 cysteine 2 mM Zn2+ protects at 2 mM cysteine, protects partially at 10 mM cysteine, no protection at 20 mM cysteine Crotalus adamanteus
3.4.24.46 EDTA 2 mM Zn2+ protects at 0.5-1 mM EDTA, no protection at 2 mM EDTA, 2 mM Ca2+ partially protects at 0.5 mM EDTA, weakly at 1 mM EDTA, no protection at 2 mM EDTA; hide powder azure or alpha1-proteinase inhibitor as substrates Crotalus adamanteus
3.4.24.46 GSH 2 mM Zn2+ or Ca2+ protects at 2 mM GSH, partially protects at 10 mM GSH, no protection at 50 mM GSH Crotalus adamanteus
3.4.24.46 additional information no inhibition by diisopropyl fluorophosphate Crotalus adamanteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.46 Ca2+ metalloproteinase Crotalus adamanteus
3.4.24.46 Zn2+ zinc containing metalloproteinase Crotalus adamanteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.46 additional information
-
 amino acid composition compared to proteinase I Crotalus adamanteus
3.4.24.46 23750
-
 Crotalus adamanteus, calculated from amino acid content Crotalus adamanteus
3.4.24.46 24000
-
 Crotalus adamanteus, PAGE Crotalus adamanteus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.46 Crotalus adamanteus
-
 Eastern diamondback rattlesnake
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.46
-
 Crotalus adamanteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.46 venom
-
 Crotalus adamanteus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.46 additional information
-
-
 Crotalus adamanteus

Storage Stability

EC Number Storage Stability Organism
3.4.24.46 -18°C, in 0.05 M sodium borate, pH 8, 2 mM CaCl2, at least 6 months Crotalus adamanteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.46 casein + H2O
-
 Crotalus adamanteus ?
-
 ?
3.4.24.46 hide powder azure + H2O
-
 Crotalus adamanteus ?
-
 ?
3.4.24.46 human plasma alpha1-proteinase inhibitor + H2O MW 54000, native and heat-denatured, the latter is more extensively hydrolyzed Crotalus adamanteus hydrolyzed and inactivated human plasma alpha1-proteinase inhibitor MW 50000 and MW 4000 fragments ?
3.4.24.46 additional information no hydrolysis of benzoyl-L-Arg ethyl ester, benzoyl-L-Tyr ethyl ester Crotalus adamanteus ?
-
 ?
3.4.24.46 Oxidized insulin B-chain + H2O cleavage at Phe1-Val2, His5-Leu6, His10-Leu11, Ala14-Leu15, Leu15-Tyr16 and Tyr16-Leu17 Crotalus adamanteus ?
-
 ?
3.4.24.46 Polyarginine + H2O
-
 Crotalus adamanteus Hydrolyzed polyarginine chain length 6-12 amino acids, not arginine monomers ?
3.4.24.46 Polylysine + H2O
-
 Crotalus adamanteus Hydrolyzed polylysine chain length 6-12 amino acids, not lysine monomers ?

Subunits

EC Number Subunits Comment Organism
3.4.24.46 monomer 1 * 24000, Crotalus adamanteus, SDS-PAGE Crotalus adamanteus