Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shannon, J.D.; Baramova, E.N.; Bjarnason, J.B.; Fox, J.W.
    Amino acid sequence of a Crotalus atrox venom metalloproteinase which cleaves type IV collagen and gelatin (1989), J. Biol. Chem., 264, 11575-11583.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.42 Collagenase inhibitor SC 44463
-
 Crotalus atrox
3.4.24.42 cysteine
-
 Crotalus atrox
3.4.24.42 EDTA
-
 Crotalus atrox
3.4.24.42 additional information iodoacetate; no inhibition by aprotinin, PMSF Crotalus atrox
3.4.24.42 phosphoramidon weak, isozyme Ht-d Crotalus atrox
3.4.24.42 thiorphan
-
 Crotalus atrox

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.1 Zn2+ zinc metalloproteinase Crotalus atrox
3.4.24.1 Zn2+ zinc binding-site Crotalus atrox
3.4.24.42 Zn2+ zinc metalloproteinase Crotalus atrox

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.1 23230
-
 Ht-d, amino acid analysis Crotalus atrox
3.4.24.42 additional information
-
 amino acid composition Crotalus atrox
3.4.24.42 additional information
-
 they differ by a single amino acid substitution at position 181, Ala (Cc) or Asp (Cd) Crotalus atrox
3.4.24.42 23230
-
 Crotalus atrox, isozyme Ht-d Crotalus atrox

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.1 Proteins + H2O Crotalus atrox preferential cleavage: -Leu, -Ile, -Val, -Phe ?
-
 ?
3.4.24.42 Basement membranes surrounding capillaries + H2O Crotalus atrox involved in disruption of capillary membranes causing hemorrhage in surrounding tissue ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.1 Crotalus atrox
-
-
-
3.4.24.42 Crotalus atrox
-
 Western diamondback rattlesnake
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.42 venom
-
 Crotalus atrox
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.1 Gelatin + H2O
-
 Crotalus atrox ?
-
 ?
3.4.24.1 additional information not: interstitial collagen type I and III Crotalus atrox ?
-
 ?
3.4.24.1 additional information specificity of Ht-d Crotalus atrox ?
-
 ?
3.4.24.1 Proteins + H2O preferential cleavage: -Leu, -Ile, -Val, -Phe Crotalus atrox ?
-
 ?
3.4.24.1 Type IV collagen + H2O
-
 Crotalus atrox ?
-
 ?
3.4.24.42 2-Aminobenzoyl-Ala-Gly-Leu-Ala 4-nitrobenzyl amide + H2O
-
 Crotalus atrox ?
-
 ?
3.4.24.42 Basement membranes surrounding capillaries + H2O involved in disruption of capillary membranes causing hemorrhage in surrounding tissue Crotalus atrox ?
-
 ?
3.4.24.42 Collagen type IV + H2O basement membrane component, alpha1 (MW 185000) and alpha2 (MW 170000) chain Crotalus atrox Hydrolyzed collagen type IV MW 170000, MW 164000, MW 125000, MW 110000, MW 940000 and MW 64000 fragments ?
3.4.24.42 Gelatin of collagen type I + H2O i.e. denatured collagen Crotalus atrox Hydrolyzed gelatin of collagen type I MW 60000 and MW 50000 fragments ?
3.4.24.42 Gelatin of collagen type III + H2O i.e. denatured collagen Crotalus atrox ?
-
 ?
3.4.24.42 Gelatin of collagen type V + H2O i.e. denatured collagen, hydrolysis at 38øC and above, not below Crotalus atrox Hydrolyzed gelatin of collagen type V MW 130000, MW 118000, MW 93000 and MW 85000 fragments ?
3.4.24.42 additional information no hydrolysis of interstitial collagen, native type I collagen Crotalus atrox ?
-
 ?