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Literature summary extracted from

  • Fox, J.W.; Campbell, R.; Beggerly, L.; Bjarnason, J.B.
    Substrate specificities and inhibition of two hemorrhagic zinc proteases Ht-c and Ht-d from Crotalus atrox venom (1986), Eur. J. Biochem., 156, 65-72.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.1 Chloromethyl esters
-
 Crotalus atrox
3.4.24.1 L-Ile-NHOH
-
 Crotalus atrox
3.4.24.1 L-Leu-CH2Cl
-
 Crotalus atrox
3.4.24.1 L-Leu-NHOH
-
 Crotalus atrox
3.4.24.1 L-Phe-NHOH
-
 Crotalus atrox
3.4.24.1 L-Val-NHOH
-
 Crotalus atrox
3.4.24.1 MeO-Suc-Gly-Leu-Phe-CH2Cl
-
 Crotalus atrox
3.4.24.1 NH2-Ala-Leu-Tyr-Leu-COOH
-
 Crotalus atrox
3.4.24.1 phosphoramidon
-
 Crotalus atrox
3.4.24.42 Ala-Leu-Tyr-Leu isozyme Ht-c Crotalus atrox
3.4.24.42 Amino acid hydroxamates isozyme Ht-c Crotalus atrox
3.4.24.42 additional information acetyl-Ala-Val-Leu-Gly-Ala, acetyl-Val-Glu-Leu-Gly-Ala, acetyl-Val-Lys-Leu-Gly-Ala, acetyl-Lys-Ala-Leu-Gly-Ala, acetyl-Val-Ala-Ile-Gly-Ala, furylacryloyl-Gly-L-Leu-NH2 Crotalus atrox
3.4.24.42 phosphoramidon i.e. N-[L-rhamnopyranosyl-oxyhydroxyphosphinyl]-L-leucyl-L-tryptophan, isozyme Ht-c Crotalus atrox

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.1 additional information
-
 additional information
-
 Crotalus atrox
3.4.24.42 0.023
-
 Val-Glu-Ala-Leu-Tyr-Leu isozyme Ht-d Crotalus atrox
3.4.24.42 0.032
-
 Leu-Val-Glu-Ala-Leu-Tyr-Leu isozyme Ht-d Crotalus atrox
3.4.24.42 0.11
-
 Leu-Val-Glu-Ala-Leu-Tyr-Leu isozyme Ht-c Crotalus atrox
3.4.24.42 0.12
-
 Val-Glu-Ala-Leu-Tyr-Leu isozyme Ht-c Crotalus atrox
3.4.24.42 0.14
-
 Glu-Ala-Leu-Tyr-Leu isozyme Ht-d Crotalus atrox
3.4.24.42 0.21
-
 acetyl-Val-Gly-Leu-Gly-Ala isozyme Ht-c Crotalus atrox
3.4.24.42 0.29
-
 Glu-Ala-Leu-Tyr-Leu isozyme Ht-c Crotalus atrox
3.4.24.42 0.31
-
 acetyl-Val-Ala-Leu-Gly-Gly isozyme c, acetyl-Val-Ala-Leu-Gly-Ala, 2-aminobenzoyl-Ala-Gly-Leu-Ala 4-nitrobenzyl amide Crotalus atrox
3.4.24.42 0.45
-
 acetyl-Val-Ala-Leu-Gly-Gly isozyme Ht-c Crotalus atrox
3.4.24.42 0.5
-
 acetyl-Val-Ala-Phe-Gly-Ala isozyme Ht-c Crotalus atrox
3.4.24.42 0.62
-
 Acetyl-Val-Ala-Leu-Leu-Ala acetyl-Val-Ala-Ala-Leu-Gly-Ala, isozyme Ht-c Crotalus atrox
3.4.24.42 0.63
-
 Acetyl-Val-Ala-Leu-Leu-Ala isozyme Ht-d Crotalus atrox
3.4.24.42 0.69
-
 Acetyl-Val-Ala-Ala-Leu-Gly-Ala isozyme Ht-d Crotalus atrox
3.4.24.42 0.71
-
 acetyl-Ala-Ala-Leu-Gly-Ala
-
 Crotalus atrox
3.4.24.42 0.76
-
 acetyl-Glu-Ala-Leu-Gly-Ala isozyme Ht-c Crotalus atrox

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.42 Zn2+ zinc metalloproteinase Crotalus atrox

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.1 Proteins + H2O Crotalus atrox preferential cleavage: -Leu, -Ile, -Val, -Phe ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.1 Crotalus atrox
-
-
-
3.4.24.42 Crotalus atrox
-
 Western diamondback rattlesnake
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.1 venom
-
 Crotalus atrox
-
3.4.24.42 venom
-
 Crotalus atrox
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.1 insulin B chain + H2O cleaves Ala14-Leu15 bond of insulin B chain most rapidly Crotalus atrox ?
-
 ?
3.4.24.1 Leu-Val-Glu-Ala-Leu-Tyr-Leu + H2O
-
 Crotalus atrox ?
-
 ?
3.4.24.1 additional information specificity of Ht-d Crotalus atrox ?
-
 ?
3.4.24.1 additional information specificity of Ht-c Crotalus atrox ?
-
 ?
3.4.24.1 Proteins + H2O preferential cleavage: -Leu, -Ile, -Val, -Phe Crotalus atrox ?
-
 ?
3.4.24.42 2-Aminobenzoyl-Ala-Gly-Leu-Ala 4-nitrobenzyl amide + H2O
-
 Crotalus atrox ?
-
 ?
3.4.24.42 acetyl-Ala-Ala-Leu-Gly-Ala + H2O
-
 Crotalus atrox ?
-
 ?
3.4.24.42 acetyl-Glu-Ala-Leu-Gly-Ala + H2O
-
 Crotalus atrox ?
-
 ?
3.4.24.42 Acetyl-Val-Ala-Ala-Leu-Gly-Ala + H2O
-
 Crotalus atrox ?
-
 ?
3.4.24.42 acetyl-Val-Ala-Leu-Gly-Gly + H2O
-
 Crotalus atrox ?
-
 ?
3.4.24.42 Acetyl-Val-Ala-Leu-Leu-Ala + H2O best substrate of synthetic acetylated pentapeptides Crotalus atrox ?
-
 ?
3.4.24.42 acetyl-Val-Ala-Phe-Gly-Ala + H2O
-
 Crotalus atrox ?
-
 ?
3.4.24.42 acetyl-Val-Gly-Leu-Gly-Ala + H2O
-
 Crotalus atrox ?
-
 ?
3.4.24.42 Glu-Ala-Leu-Tyr-Leu + H2O peptide derived from insulin B-chain Crotalus atrox ?
-
 ?
3.4.24.42 Leu-Val-Glu-Ala-Leu-Tyr-Leu + H2O peptide derived from insulin B-chain, best substrate Crotalus atrox Leu-Val-Glu-Ala + Leu-Tyr-Leu
-
 ?
3.4.24.42 additional information no hydrolysis of peptide Ala-Leu-Tyr-Leu Crotalus atrox ?
-
 ?
3.4.24.42 additional information cleavage specificity, compared to hemorrhagic toxins a and b Crotalus atrox ?
-
 ?
3.4.24.42 additional information substrate requirements: small aliphatic residues at P1 (Ala or Gly) and Leu at P2 Crotalus atrox ?
-
 ?
3.4.24.42 Oxidized insulin B-chain + H2O cleavage at Gly23-Phe24 Crotalus atrox ?
-
 ?
3.4.24.42 Oxidized insulin B-chain + H2O cleavage at His5-Leu6, His10-Leu11, Ala14-Leu15 (most rapidly cleaved bond) Crotalus atrox ?
-
 ?
3.4.24.42 Oxidized insulin B-chain + H2O cleavage at Tyr16-Leu17 (slightly less rapidly cleaved bond) Crotalus atrox ?
-
 ?
3.4.24.42 Val-Glu-Ala-Leu-Tyr-Leu + H2O peptide derived from insulin B-chain Crotalus atrox ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.42 37
-
 assay at Crotalus atrox