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Literature summary extracted from

  • Morihara, K.
    Pseudomonas aeruginosa proteinase. I. Purification and general properties (1963), Biochim. Biophys. Acta, 73, 113-124.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.40 Pseudomonas aeruginosa Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.40 1,10-phenanthroline 10 mM; strong Pseudomonas aeruginosa
3.4.24.40 2,2'-bipyridyl not Pseudomonas aeruginosa
3.4.24.40 Cu2+
-
 Pseudomonas aeruginosa
3.4.24.40 EDTA Co2+; Mn2+; strong, 5 mM Pseudomonas aeruginosa
3.4.24.40 Fe2+ strong Pseudomonas aeruginosa
3.4.24.40 Hg2+
-
 Pseudomonas aeruginosa
3.4.24.40 KCN not Pseudomonas aeruginosa
3.4.24.40 KMnO4
-
 Pseudomonas aeruginosa
3.4.24.40 additional information citrate, NaF, oxalate; diisopropyl phosphofluoridate; monoiodoacetic acid; PCMB; potato inhibitor; soybean trypsin inhibitor Pseudomonas aeruginosa
3.4.24.40 N-bromosuccinimide
-
 Pseudomonas aeruginosa
3.4.24.40 Pb2+
-
 Pseudomonas aeruginosa
3.4.24.40 Sodium thioglycolate not Pseudomonas aeruginosa
3.4.24.40 Urea
-
 Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.40 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.40
-
 Pseudomonas aeruginosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.40 culture supernatant
-
 Pseudomonas aeruginosa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.40 casein + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Egg albumin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Gelatin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 Hemoglobin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 additional information benzoyl-L-Arg amide, acetyl-L-Tyr ethyl ester, carbobenzoxy-Glu-Tyr, carbobenzoxy-Gly-Phe, L-Leu amide Pseudomonas aeruginosa ?
-
 ?
3.4.24.40 additional information collagen Pseudomonas aeruginosa ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.40 60
-
-
 Pseudomonas aeruginosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.24.40 42 62 about half-maximal activity at 42°C and 62°C Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.40 30
-
 pH 5-9, fairly stable Pseudomonas aeruginosa
3.4.24.40 50
-
 below, 10 min, fairly stable at neutral pH-values Pseudomonas aeruginosa
3.4.24.40 55
-
 10 min, partly unstable in distilled water, 100 mM phosphate or 10 mM CaCl2 stabilizes Pseudomonas aeruginosa
3.4.24.40 63
-
 10 min, inactivation in the presence of stabilizing agents Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.40 additional information
-
 pI: 4 Pseudomonas aeruginosa
3.4.24.40 7 9 egg white albumin, hemoglobin as substrates Pseudomonas aeruginosa
3.4.24.40 8 9 casein as substrate Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.40 6 10 about half-maximal activity at pH 6 and about 80% of maximal activity at pH 10, casein as substrate, about 75% of maximal activity at pH 6 and about 60% of maximal activity at pH 10, egg white albumin as substrate Pseudomonas aeruginosa
3.4.24.40 6.5 10 about half-maximal activity at pH 6.5 and about 65% of maximal activity at pH 10, hemoglobin as substrate Pseudomonas aeruginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.40 3.5 11 stable in this range Pseudomonas aeruginosa
3.4.24.40 5 9 10 min, fairly stable at 30°C Pseudomonas aeruginosa