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Literature summary extracted from

  • Morihara, K.; Tsuzuki, H.; Oka, T.
    On the specificity of Pseudomonas aeruginosa alkaline proteinase with synthetic peptides (1973), Biochim. Biophys. Acta, 309, 414-429.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.40 benzyloxycarbonyl-D-Ala-Leu-Ala competitive to benzyloxycarbonyl-Ala-Phe-Gly-Ala Pseudomonas aeruginosa
3.4.24.40 Benzyloxycarbonyl-Gly-Leu-D-Ala benzyloxycarbonyl-Ala-Phe-Gly-Ala as substrate Pseudomonas aeruginosa
3.4.24.40 Benzyloxycarbonyl-Gly-Leu-Gly benzyloxycarbonyl-Ala-Phe-Gly-Ala as substrate Pseudomonas aeruginosa
3.4.24.40 Benzyloxycarbonyl-Gly-Leu-NH2 benzyloxycarbonyl-Ala-Phe-Gly-Ala as substrate Pseudomonas aeruginosa
3.4.24.40 Benzyloxycarbonyl-Phe-D-Leu-Ala competitive to benzyloxycarbonyl-Ala-Phe-Gly-Ala Pseudomonas aeruginosa
3.4.24.40 additional information diisopropyl phosphofluoridate; PCMB; potato inhibitor; soybean trypsin inhibitor; tosyl-L-Phe chloromethyl ketone or tosyl-L-Lys chloromethyl ketone Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.40 0.2
-
benzyloxycarbonyl-Phe-Phe-Ala
-
Pseudomonas aeruginosa
3.4.24.40 0.4
-
Benzyloxycarbonyl-Phe-Ala-Ala benzyloxycarbonyl-Phe-Leu-Ala Pseudomonas aeruginosa
3.4.24.40 0.5
-
Ala-Ala-Ala-Ala-Ala-Ala benzyloxycarbonyl-Phe-L-Ala-Ala Pseudomonas aeruginosa
3.4.24.40 0.6
-
benzyloxycarbonyl-Gly-Leu-Phe benzyloxycarbonyl-Ala-Phe-Leu-Ala Pseudomonas aeruginosa
3.4.24.40 0.9
-
Benzyloxycarbonyl-Gly-Leu-Leu benzyloxycarbonyl-Gly-Phe-Leu-Ala Pseudomonas aeruginosa
3.4.24.40 1.2
-
Ala-Ala-Ala-Ala-Ala
-
Pseudomonas aeruginosa
3.4.24.40 1.5
-
benzyloxycarbonyl-Ala-Leu-Ala
-
Pseudomonas aeruginosa
3.4.24.40 1.8
-
benzyloxycarbonyl-Phe-Gly-Ala benzyloxycarbonyl-Phe-Ser-Ala, benzyloxycarbonyl-Leu-Ala-Ala Pseudomonas aeruginosa
3.4.24.40 2.3
-
benzyloxycarbonyl-Phe-Gly-Leu-Ala benzyloxycarbonyl-Ala-Leu-Gly-Gly Pseudomonas aeruginosa
3.4.24.40 2.4
-
benzyloxycarbonyl-Gly-Leu-Gly-Ala
-
Pseudomonas aeruginosa
3.4.24.40 2.5
-
benzyloxycarbonyl-Val-Ala-Ala benzyloxycarbonyl-Ala-Phe-Gly-Ala Pseudomonas aeruginosa
3.4.24.40 2.7
-
Benzyloxycarbonyl-Ala-Ala-Ala
-
Pseudomonas aeruginosa
3.4.24.40 2.9
-
Benzyloxycarbonyl-Gly-Leu-Ala
-
Pseudomonas aeruginosa
3.4.24.40 4.3
-
Benzyloxycarbonyl-Gly-Leu-Gly-Gly-Ala
-
Pseudomonas aeruginosa
3.4.24.40 4.8
-
benzyloxycarbonyl-Ala-Gly-Leu-Ala
-
Pseudomonas aeruginosa
3.4.24.40 5.3
-
Benzyloxycarbonyl-Gly-Pro-Gly-Gly-Pro-Ala
-
Pseudomonas aeruginosa
3.4.24.40 5.4
-
benzyloxycarbonyl-Gly-Gly-Leu-Ala benzyloxycarbonyl-Ala-Gly-Gly-Leu Pseudomonas aeruginosa
3.4.24.40 7.7
-
Benzyloxycarbonyl-Gly-Ala-Ala
-
Pseudomonas aeruginosa
3.4.24.40 8.7
-
Ala-Ala-Ala-Ala
-
Pseudomonas aeruginosa
3.4.24.40 11
-
Benzyloxycarbonyl-Gly-Leu-Gly-Gly
-
Pseudomonas aeruginosa
3.4.24.40 17.3
-
Phe-Gly-Leu-Ala
-
Pseudomonas aeruginosa
3.4.24.40 20
-
benzyloxycarbonyl-D-Ala-Gly-Leu-Ala
-
Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.40 Ca2+ no activation by added Ca2+ Pseudomonas aeruginosa
3.4.24.40 Ca2+ 1-2 gatom/mol enzyme Pseudomonas aeruginosa
3.4.24.40 Co2+ activation Pseudomonas aeruginosa
3.4.24.40 Co2+ above 5 mM, benzoxycarbonyl-Ala-Phe-Gly-Ala as substrate Pseudomonas aeruginosa
3.4.24.40 additional information contains no significant amount of any metal except Ca2+ Pseudomonas aeruginosa
3.4.24.40 additional information no activation by Mn2+ Pseudomonas aeruginosa
3.4.24.40 additional information no activation by Ni2+ Pseudomonas aeruginosa
3.4.24.40 Zn2+ zinc-containing metalloproteinase (not) Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.40 48400
-
Pseudomonas aeruginosa, sedimentation velocity centrifugation Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.40 Pseudomonas aeruginosa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.40 Benzyloxycarbonyl tripeptides + H2O overview Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Ala-Ala-Ala + H2O cleavage site: AlaÄ+ÄAla-Ala Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Ala-Gly-Gly-Leu-Ala + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Ala-Gly-Gly-Leu-Xaa + H2O a hydrophobic or bulky residue at P3' results in marked increase in hydrolysis Pseudomonas aeruginosa ?
-
?
3.4.24.40 benzyloxycarbonyl-Ala-Gly-Leu-Ala + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 benzyloxycarbonyl-Ala-Leu-Ala + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 benzyloxycarbonyl-D-Ala-Gly-Leu-Ala + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Gly-Ala-Ala + H2O cleavage site: Gly-Ala Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Gly-Gly-Gly-Leu + H2O poor substrate Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Gly-Gly-Leu amide + H2O poor substrate Pseudomonas aeruginosa ?
-
?
3.4.24.40 benzyloxycarbonyl-Gly-Gly-Leu-Ala + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Gly-Leu-Ala + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 benzyloxycarbonyl-Gly-Leu-Gly-Ala + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Gly-Leu-Gly-Gly + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Gly-Leu-Gly-Gly-Ala + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Gly-Leu-Gly-Gly-Xaa + H2O a hydrophobic or bulky residue at P3' results in marked increase in hydrolysis Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Gly-Leu-Leu + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Gly-Leu-Phe + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Gly-Leu-Xaa + H2O at position Xaa with decreasing susceptibility to the enzyme: Leu, Phe or Ala, poor substrates: Gly or NH2, D-Ala Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Gly-Pro-Gly-Gly-Pro-Ala + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Phe-Ala-Ala + H2O cleavage site: Phe-Ala Pseudomonas aeruginosa ?
-
?
3.4.24.40 benzyloxycarbonyl-Phe-Gly-Ala + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 benzyloxycarbonyl-Phe-Gly-Leu-Ala + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Phe-Leu-Ala-Ala + H2O best substrate Pseudomonas aeruginosa ?
-
?
3.4.24.40 benzyloxycarbonyl-Phe-Phe-Ala + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Phe-Xaa-Ala + H2O susceptibility to the enzyme is Xaa-dependent, in decreasing order of efficiency: Ala, Phe, Leu, Trp or Ser or Gly Pseudomonas aeruginosa Benzyloxycarbonyl-Phe + Xaa-Ala
-
?
3.4.24.40 benzyloxycarbonyl-Val-Ala-Ala + H2O
-
Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Xaa-Ala-Ala + H2O susceptibility to the enzyme is Xaa-dependent, in decreasing order of efficiency: L-Phe, Leu or Ala, Val, Gly, D-Ala Pseudomonas aeruginosa Benzyloxycarbonyl-Xaa + Ala-Ala
-
?
3.4.24.40 Benzyloxycarbonyl-Xaa-Gly-Leu-Ala + H2O a hydrophobic or bulky residue at P2 results in marked increase in hydrolysis Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Xaa-Leu-Ala + H2O susceptibility to the enzyme is Xaa-dependent, in decreasing order of efficiency: L-Phe, Leu or Ala, Val, Gly, D-Ala Pseudomonas aeruginosa Benzyloxycarbonyl-Xaa + Leu-Ala
-
?
3.4.24.40 Benzyloxycarbonyl-Xaa-Phe-Gly-Ala + H2O a hydrophobic or bulky residue at P2 results in marked increase in hydrolysis Pseudomonas aeruginosa ?
-
?
3.4.24.40 Benzyloxycarbonyl-Xaa-Phe-Leu-Ala + H2O a hydrophobic or bulky residue at P2 results in marked increase in hydrolysis Pseudomonas aeruginosa ?
-
?
3.4.24.40 L-Ala-L-Ala-L-Ala-L-Ala + H2O
-
Pseudomonas aeruginosa L-Ala-L-Ala + L-Ala-L-Ala
-
?
3.4.24.40 L-Ala-L-Ala-L-Ala-L-Ala-L-Ala + H2O
-
Pseudomonas aeruginosa L-Ala-L-Ala + L-Ala-L-Ala-L-Ala
-
?
3.4.24.40 L-Ala-L-Ala-L-Ala-L-Ala-L-Ala-L-Ala + H2O
-
Pseudomonas aeruginosa L-Ala-L-Ala-L-Ala + L-Ala-L-Ala-L-Ala
-
?
3.4.24.40 L-Ala-oligopeptides + H2O proteolytic activity increases drastically with increasing chain length from tetramer to hexamer, no substrate: dipeptide or tripeptide Pseudomonas aeruginosa ?
-
?
3.4.24.40 additional information elastin Pseudomonas aeruginosa ?
-
?
3.4.24.40 additional information tetrapeptides are poorer substrates than benzyloxycarbonyl tetrapeptides Pseudomonas aeruginosa ?
-
?
3.4.24.40 additional information benzyloxycarbonyl dipeptides, N-acetylated amino acid esters or amides, dipeptide amides, tripeptides, benzyloxycarbonyl-(Gly)4, benzyloxycarbonyl-(Gly)5, benzyloxycarbonyl-Gly-Leu-Gly-Gly-D-Ala Pseudomonas aeruginosa ?
-
?
3.4.24.40 Oxidized insulin B-chain + H2O cleavage sites Pseudomonas aeruginosa ?
-
?
3.4.24.40 Phe-Gly-Leu-Ala + H2O
-
Pseudomonas aeruginosa ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.40 additional information
-
pI: 4 Pseudomonas aeruginosa
3.4.24.40 7
-
benzyloxycarbonyl-Phe-Ala-Ala or benzyloxycarbonyl-Ala-Phe-Gly-Ala as substrate Pseudomonas aeruginosa