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Literature summary extracted from

  • Jaenicke, L.; Kuhne, W.; Spessert, R.; Wahle, U.; Waffenschmidt, S.
    Cell-wall lytic enzymes (autolysins) of Chlamydomonas reinhardtii are (hydroxy)proline-specific proteases (1987), Eur. J. Biochem., 170, 485-491.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.38 1,10-phenanthroline 0.2 mM, strong Chlamydomonas reinhardtii
3.4.24.38 2,2'-dipyridyl 2 mM Chlamydomonas reinhardtii
3.4.24.38 Aprotinin
-
 Chlamydomonas reinhardtii
3.4.24.38 cysteine
-
 Chlamydomonas reinhardtii
3.4.24.38 EDTA 0.4 mM, partially reversed by Ca2+ or Mg2+ Chlamydomonas reinhardtii
3.4.24.38 histidine
-
 Chlamydomonas reinhardtii
3.4.24.38 imidazole
-
 Chlamydomonas reinhardtii
3.4.24.38 Lectin from Canavalia ensiformis not from Ricinus communis Chlamydomonas reinhardtii
3.4.24.38 additional information chymostatin, antipain, leupeptin; pepstatin A Chlamydomonas reinhardtii
3.4.24.38 phosphoramidon weak Chlamydomonas reinhardtii
3.4.24.38 PMSF
-
 Chlamydomonas reinhardtii
3.4.24.38 tosyl-Lys chloromethyl ketone 2 mM, less efficient than tosyl-Phe-chloromethyl ketone Chlamydomonas reinhardtii
3.4.24.38 tosyl-Phe chloromethyl ketone i.e. TPCK, 2 mM Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.38 67000
-
 Chlamydomonas reinhardtii, gel filtration Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.38 Chlamydomonas reinhardtii
-
 green alga, wild-type strain 137c
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.38 glycoprotein
-
 Chlamydomonas reinhardtii

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.38
-
 Chlamydomonas reinhardtii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.38 additional information isolated from gametes Chlamydomonas reinhardtii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.38 additional information
-
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.38 Azocoll + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
3.4.24.38 Gelatin + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
3.4.24.38 Heat-denatured collagen + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
3.4.24.38 Leu-Trp-Met-Arg-Phe-Ala + H2O
-
 Chlamydomonas reinhardtii Leu-Trp-Met + Arg-Phe-Ala + Leu-Trp + Met-Arg-Phe-Ala
-
 ?
3.4.24.38 additional information bovine serum albumin Chlamydomonas reinhardtii ?
-
 ?
3.4.24.38 additional information hemoglobin, casein, poly(Pro) or poly(hydroxyproline) Chlamydomonas reinhardtii ?
-
 ?
3.4.24.38 additional information Chlamydomonas reinhardtii has a second cell wall lytic enzyme, a sporangial autolysin Chlamydomonas reinhardtii ?
-
 ?
3.4.24.38 Proteins rich in proline and hydroxyproline + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
3.4.24.38 Zoosporangia cell walls + H2O selected domains within walls of gametes, zoospores or zoosporangia Chlamydomonas reinhardtii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.38 monomer 1 * 67000, Chlamydomonas reinhardtii, SDS-PAGE Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
3.4.24.38 Gamete autolysin
-
 Chlamydomonas reinhardtii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.38 50
-
 inactivation Chlamydomonas reinhardtii
3.4.24.38 50
-
 30 min Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.38 9
-
 broad Chlamydomonas reinhardtii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.38 6 11 about half-maximal activity at pH 6 and 11, about 80% of maximal activity at pH 7 and 10.5 Chlamydomonas reinhardtii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.38 9
-
 several h stable Chlamydomonas reinhardtii