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Literature summary extracted from

  • Hasty, K.A.; Jeffrey, J.J.; Hibbs, M.S.; Welgus, H.G.
    The collagen substrate specificity of human neutrophil collagenase (1987), J. Biol. Chem., 262, 10048-10052.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.34 0.0006
-
 guinea pig collagen type I 37°C Homo sapiens
3.4.24.34 0.0007
-
 human collagen type I 37°C Homo sapiens
3.4.24.34 0.0011
-
 human collagen type II 37°C Homo sapiens
3.4.24.34 0.0018
-
 human collagen type III 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.34 specific granule
-
 Homo sapiens 42581
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.34 Collagen + H2O Homo sapiens initiates degradation of native collagen ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.34 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.34 from culture supernatant of phorbol myristate acetate stimulated neutrophils, immunoaffinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.34 leukocyte neutrophil Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.34 additional information
-
 0.13 mg fibrillar guinea pig collagen type I/mg enzyme/min, 0.09 mg human collagen type III/mg enzyme/min, 25°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.34 Collagen + H2O initiates degradation of native collagen Homo sapiens ?
-
 ?
3.4.24.34 Fibrillar type I collagen + H2O from human or guinea pig Homo sapiens ?
-
 ?
3.4.24.34 Fibrillar type III collagen + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.34 guinea pig collagen type I + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.34 human collagen type I + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.34 human collagen type II + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.34 human collagen type III + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.34 Monomeric type I collagen + H2O from human Homo sapiens collagen type I fragment TCA + collagen type I fragment TCB
-
 ?
3.4.24.34 Monomeric type I collagen + H2O from guinea pig Homo sapiens collagen type I fragment TCA + collagen type I fragment TCB
-
 ?
3.4.24.34 Monomeric type I collagen + H2O best substrate, unlike EC 3.4.24.7 this enzyme cleaves type III collagen more slowly than type I Homo sapiens collagen type I fragment TCA + collagen type I fragment TCB
-
 ?
3.4.24.34 Monomeric type I collagen + H2O catalyzes single cleavage in alpha-chain Homo sapiens collagen type I fragment TCA + collagen type I fragment TCB
-
 ?
3.4.24.34 Monomeric type II collagen + H2O
-
 Homo sapiens collagen type II fragment TCA + collagen type II fragment TCB three-quarter and one-quarter length fragments ?
3.4.24.34 Monomeric type III collagen + H2O unlike EC 3.4.24.7 this enzyme cleaves type III collagen more slowly than type I Homo sapiens collagen type III fragment TCA + collagen type III fragmentTCB three-quarter and one-quarter length fragments ?
3.4.24.34 additional information no hydrolysis of monomeric type IV or V collagen Homo sapiens ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.34 25
-
 assay at Homo sapiens
3.4.24.34 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.34 0.000233
-
 human collagen type III 37°C Homo sapiens
3.4.24.34 0.00065
-
 human collagen type II 37°C Homo sapiens
3.4.24.34 0.00161
-
 guinea pig collagen type I 37°C Homo sapiens
3.4.24.34 0.00178
-
 human collagen type I 37°C Homo sapiens