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Literature summary extracted from

  • Arvidson, S.; Holme, T.; Lindholm, B.
    Studies on extracellular proteolytic enzymes from Staphylococcus aureus. I. Purification and characterization of one neutral and one alkaline protease (1973), Biochim. Biophys. Acta, 302, 135-148.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.29 Reducing agents protease II active only in presence of reducing agents such as cysteine, 2-mercaptoethanol or sodium thioglycollate Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.29 Ag2+ protease II Staphylococcus aureus
3.4.24.29 Co2+ protease II inactive in presence of, protease I slightly stimulated Staphylococcus aureus
3.4.24.29 Cu2+ protease II Staphylococcus aureus
3.4.24.29 DFP 1.0 mM, 30 min, 20°C, activity of protease I is reduced by 10%, activity of protease II by 30% Staphylococcus aureus
3.4.24.29 Hg2+ protease II Staphylococcus aureus
3.4.24.29 Mn2+ protease II Staphylococcus aureus
3.4.24.29 additional information protease I is not significantly affected by SH-reducing, SH-inactivating or metal-complexing agents Staphylococcus aureus
3.4.24.29 NaCl 50% reduction of activity at 0.3 M for protease I and 0.5 M for protease II Staphylococcus aureus
3.4.24.29 Zn2+ protease II Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.29 extracellular
-
 Staphylococcus aureus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.29 Ca2+ slight stimulation of protease I Staphylococcus aureus
3.4.24.29 Co2+ slight stimulation of protease I, protease II inactive in presence of Staphylococcus aureus
3.4.24.29 Mg2+ slight stimulation of protease I Staphylococcus aureus
3.4.24.29 Pb2+ slight stimulation of protease I Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.29 12500
-
 Staphylococcus aureus, protease II, gel filtration Staphylococcus aureus
3.4.24.29 21000
-
 Staphylococcus aureus, protease I, gel filtration Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.29 Staphylococcus aureus
-
-
-
3.4.24.29 Staphylococcus aureus V8
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.29 protease I (pI: 4.0) and protease II (pI: 9.4) Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.29 additional information
-
-
 Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.29 casein + H2O
-
 Staphylococcus aureus hydrolyzed casein
-
 ?
3.4.24.29 casein + H2O
-
 Staphylococcus aureus V8 hydrolyzed casein
-
 ?
3.4.24.29 Hemoglobin + H2O
-
 Staphylococcus aureus Hydrolyzed hemoglobin
-
 ?
3.4.24.29 Hemoglobin + H2O
-
 Staphylococcus aureus V8 Hydrolyzed hemoglobin
-
 ?
3.4.24.29 additional information protease II exhibits esterase activity, using N-benzoyl-L-Tyr ethyl ester as substrate, no activity of protease I Staphylococcus aureus ?
-
 ?
3.4.24.29 additional information protease II exhibits esterase activity, using N-benzoyl-L-Tyr ethyl ester as substrate, no activity of protease I Staphylococcus aureus V8 ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.29 37
-
 pH 4.0-11.0, 3 h, protease I stable Staphylococcus aureus
3.4.24.29 45
-
 pH 7.0, 30 min, stable up to, protease I Staphylococcus aureus
3.4.24.29 50
-
 pH 7.0, 30 min, 90% loss of activity, protease II Staphylococcus aureus
3.4.24.29 90
-
 pH 7.0, 30 min, complete inactivation, protease I Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.29 5
-
 and 7.0-9.0, hemoglobin, protease I Staphylococcus aureus
3.4.24.29 7 9 and 5.0, hemoglobin, protease I Staphylococcus aureus
3.4.24.29 7.8
-
 casein, protease I Staphylococcus aureus
3.4.24.29 8.8
-
 casein, protease II Staphylococcus aureus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.29 4 11 37°C, 3 h, protease I stable Staphylococcus aureus
3.4.24.29 7
-
 37°C, 3 h, 47% loss of activity, protease II Staphylococcus aureus
3.4.24.29 8
-
 37°C, 3 h, 74% loss of activity, protease II Staphylococcus aureus