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Literature summary extracted from

  • Wilkes, S.H.; Prescott, J.M.
    Aeromonas neutral protease (1976), Methods Enzymol., 45, 404-415.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.24.25 Lyophilization and reconstitution results in approximately 20% loss of activity Vibrio proteolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.25 3.5
-
 Furylacryloyl-Gly-Leu-NH2
-
 Vibrio proteolyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.25 Calcium contains 1.7 mol of calcium per mol of enzyme, after 48 h of dialysis, not essential for activity, Ca2+ likely plays a structural role Vibrio proteolyticus
3.4.24.25 Zinc essential for activity Vibrio proteolyticus
3.4.24.25 Zinc enzyme contains 1 mol of zinc per mol of enzyme Vibrio proteolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.25 34800
-
 Aeromonas proteolytica, sedimentation equilibrium, sedimentation velocity-diffusion experiments Vibrio proteolyticus
3.4.24.25 35000
-
 1 * 35000 (about), Aeromonas proteolytica, sedimentation equilibrium in presence of guanidine-HCl Vibrio proteolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.25 Vibrio proteolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.25
-
 Vibrio proteolyticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.25 culture filtrate
-
 Vibrio proteolyticus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.25 additional information
-
-
 Vibrio proteolyticus

Storage Stability

EC Number Storage Stability Organism
3.4.24.25 -20°C, Tricine or Tris buffer, stable for several years Vibrio proteolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.25 casein + H2O
-
 Vibrio proteolyticus ?
-
 ?
3.4.24.25 furylacryloyl-Gly-Leu-NH2 + H2O
-
 Vibrio proteolyticus furylacryloyl-Gly + Leu-NH2
-
 ?
3.4.24.25 Gelatin + H2O
-
 Vibrio proteolyticus ?
-
 ?
3.4.24.25 additional information preference for bonds of the type X-Phe, X-Leu, X-Tyr to which a hydrophobic residue contributes the amino group, and whereas it is essential that the NH2-terminal position of dipeptide substrates be blocked, the enzyme hydrolyzes substrates with free carboxyl groups Vibrio proteolyticus ?
-
 ?
3.4.24.25 Urea-denatured hemoglobin + H2O
-
 Vibrio proteolyticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.25 monomer 1 * 35000 (about), Aeromonas proteolytica, sedimentation equilibrium in presence of guanidine-HCl Vibrio proteolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.25 25
-
 or 37°C, assay at Vibrio proteolyticus
3.4.24.25 37
-
 or 25°C, assay at Vibrio proteolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.25 8
-
-
 Vibrio proteolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.25 7 11 10°C, stable for more than 1 week Vibrio proteolyticus