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Literature summary extracted from

  • Stöcker, W.; Wolz, R.L.; Zwilling, R.; Strydom, D.J.; Auld, D.S.
    Astacus protease, a zinc metalloenzyme (1988), Biochemistry, 27, 5026-5032.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.21 1,10-phenanthroline r; reactivation of apoenzyme by Zn2+ (100%), Cu2+ (70%) or Co2+ (50%); strong, kinetics Astacus astacus
3.4.24.21 1,7-phenanthroline weak Astacus astacus
3.4.24.21 2,2'-dipyridyl r Astacus astacus
3.4.24.21 8-Hydroxyquinoline-5-sulfonic acid r Astacus astacus
3.4.24.21 dipicolinic acid r Astacus astacus
3.4.24.21 EDTA r (upon dilution or addition of Zn2+); weak, kinetics, t1/2 of enzyme activity at 5 mM EDTA: 6 days Astacus astacus
3.4.24.21 additional information inhibition study Astacus astacus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.21 soluble
-
 Astacus astacus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.21 additional information metal content Astacus astacus
3.4.24.21 Zn2+ requirement Astacus astacus
3.4.24.21 Zn2+ zinc metalloenzyme Astacus astacus
3.4.24.21 Zn2+ mechanism Astacus astacus
3.4.24.21 Zn2+ about 1 mol zinc per mol enzyme, atomic absorption spectroscopy Astacus astacus
3.4.24.21 Zn2+ very tightly bound, 40 days half-life for metal dissociation Astacus astacus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.21 Astacus astacus
-
 European freshwater crayfish
-
3.4.24.21 Astacus astacus
-
 i.e. Astacus fluviatilis
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.21 cardia
-
 Astacus astacus
-
3.4.24.21 digestive juice
-
 Astacus astacus
-

Storage Stability

EC Number Storage Stability Organism
3.4.24.21 4°C, apoenzyme, at least 1 month Astacus astacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.21 Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O
-
 Astacus astacus Succinyl-Ala-Ala-Ala + p-nitroaniline
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.21 25
-
 assay at Astacus astacus