EC Number | General Stability | Organism |
---|---|---|
3.4.24.20 | Stable to 5 h at 37°C in 0.1 M acetate buffer, pH 5, containing 2 M urea, 1 M guanidine-HCl or 0.01% SDS, inactivation at higher concentrations of urea, guanidine-HCl or SDS | Pleurotus ostreatus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.20 | 1,10-phenanthroline | - |
Pleurotus ostreatus | |
3.4.24.20 | 2,2'-bipyridine | 1 mM, weak | Pleurotus ostreatus | |
3.4.24.20 | 2-mercaptoethanol | not | Pleurotus ostreatus | |
3.4.24.20 | Ag2+ | 1 mM, strong | Pleurotus ostreatus | |
3.4.24.20 | antipain | not | Pleurotus ostreatus | |
3.4.24.20 | Cu2+ | 1 mM, caseinolytic activity; strong | Pleurotus ostreatus | |
3.4.24.20 | dithiothreitol | caseinolytic activity; weak | Pleurotus ostreatus | |
3.4.24.20 | EDTA | 50 mM, weak at 1 mM; fully restorable by Zn2+, Mn2+, Co2+ | Pleurotus ostreatus | |
3.4.24.20 | Guanidine-HCl | 2 M and above, not at 1 M | Pleurotus ostreatus | |
3.4.24.20 | Hg2+ | 1 mM; strong, caseinolytic activity | Pleurotus ostreatus | |
3.4.24.20 | additional information | L-cysteine; no inhibition (of caseinolytic activity) by chymostatin, iodoacetate, leupeptin; PMSF | Pleurotus ostreatus | |
3.4.24.20 | N-ethylmaleimide | weak, caseinolytic activity | Pleurotus ostreatus | |
3.4.24.20 | p-chloromercuribenzoate | weak, caseinolytic activity | Pleurotus ostreatus | |
3.4.24.20 | phosphoramidon | not | Pleurotus ostreatus | |
3.4.24.20 | SDS | 0.1% and above, not at 0.01% | Pleurotus ostreatus | |
3.4.24.20 | tosyl-Lys chloromethyl ketone | weak, caseinolytic | Pleurotus ostreatus | |
3.4.24.20 | Urea | 5 M, not at 2 M | Pleurotus ostreatus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.20 | cytosol | - |
Pleurotus ostreatus | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.20 | Zinc | requirement, 1 atom per molecule of native enzyme | Pleurotus ostreatus | |
3.4.24.20 | Zinc | atomic absorption spectroscopy | Pleurotus ostreatus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.20 | 18000 | - |
1 * 18000, Pleurotus ostreatus, SDS-PAGE | Pleurotus ostreatus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.20 | Pleurotus ostreatus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.20 | - |
Pleurotus ostreatus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.24.20 | fruit body | - |
Pleurotus ostreatus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.24.20 | additional information | - |
- |
Pleurotus ostreatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.20 | azocasein + H2O | - |
Pleurotus ostreatus | ? | - |
? | |
3.4.24.20 | Bovine insulin B-chain + H2O | cleavage site: Pro29-Lys30, yielding 2 fragments: dipeptide Lys-Ala and substrate devoid of 2 C-terminal amino acids | Pleurotus ostreatus | ? | - |
? | |
3.4.24.20 | Equine heart cytochrome c + H2O | - |
Pleurotus ostreatus | ? | - |
? | |
3.4.24.20 | Polyarginine + H2O | - |
Pleurotus ostreatus | ? | - |
? | |
3.4.24.20 | Polylysine + H2O | best substrate | Pleurotus ostreatus | ? | - |
? | |
3.4.24.20 | protamine sulfate + H2O | - |
Pleurotus ostreatus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.20 | monomer | 1 * 18000, Pleurotus ostreatus, SDS-PAGE | Pleurotus ostreatus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.20 | 37 | - |
assay at | Pleurotus ostreatus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.20 | 50 | - |
15 min, 10% loss of caseinolytic activity | Pleurotus ostreatus |
3.4.24.20 | 70 | - |
15 min, inactivation | Pleurotus ostreatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.20 | additional information | - |
pI: 8.35 | Pleurotus ostreatus |
3.4.24.20 | 5.6 | - |
azocoll or azocasein as substrate | Pleurotus ostreatus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.20 | 4 | 9 | 18 h, 10% loss of caseinolytic activity at 4°C | Pleurotus ostreatus |