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Literature summary extracted from

  • Njieha, F.K.; Morikawa, T.; Tuderman, L.; Prockop, D.J.
    Partial purification of a procollagen C-proteinase. Inhibition by synthetic peptides and sequential cleavage of type I procollagen (1982), Biochemistry, 21, 757-764.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.19 1,10-phenanthroline
-
 Gallus gallus
3.4.24.19 2,2'-dipyridyl
-
 Gallus gallus
3.4.24.19 DTT
-
 Gallus gallus
3.4.24.19 EDTA
-
 Gallus gallus
3.4.24.19 Fetal calf serum
-
 Gallus gallus
3.4.24.19 metal chelators
-
 Gallus gallus
3.4.24.19 additional information not: cysteine proteinases; not: inhibitors of serine Gallus gallus
3.4.24.19 Synthetic peptides with amino acid sequences similar to the amino acid sequence around the sites at which the C-propeptides are cleaved during the conversion of procollagen to collagen in vivo, e.g. Tyr-Tyr-Arg-Ala-Asp-Asp-Ala, 35-60% inhibition at 6-12 mM, shorter peptides containing the Ala-Asp bond cleaved by the enzyme are less effective Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.19 80000
-
 gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.19 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.19 partial Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.19 bone membranous bones of chick embryos Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.19 additional information
-
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.19 additional information in a reaction with modified procollagen the enzyme preferentially cleaves the C-propeptides from the pro-alpha chains in the order pro-alpha1, pro-alpha1 and then pro-alpha2 Gallus gallus ?
-
 ?
3.4.24.19 Procollagen type I + H2O
-
 Gallus gallus Type I pNcollagen + C-propeptides
-
 ?
3.4.24.19 Procollagen type II + H2O
-
 Gallus gallus Type II pNcollagen + C-propeptides
-
 ?
3.4.24.19 Procollagen type III + H2O
-
 Gallus gallus Type III pNcollagen + C-propeptides
-
 ?